Mahan S M, Andrew H R, Tebele N, Burridge M J, Barbet A F
University of Florida/USAID/SADC Heartwater Research Project, Veterinary Laboratory Diagnostic and Research Branch, Causeway, Zimbabwe.
Res Vet Sci. 1995 Jan;58(1):46-9. doi: 10.1016/0034-5288(95)90087-x.
The immunisation of sheep with inactivated Cowdria ruminantium organisms (the causative agent of heartwater) emulsified in Freund's adjuvant induced protective immunity against a homologous challenge with virulent cell culture-derived C ruminantium organisms. This protective immunity was associated with the development of high titres of C ruminantium-specific antibodies, a low level of rickettsiosis in brain endothelium and no mortalities. In contrast, when unimmunised sheep were challenged, they developed higher levels of rickettsiosis in brain endothelium, and some of them died. This method of immunisation against heartwater has the potential to replace the infection and treatment method that is in current use.
用弗氏佐剂乳化的灭活反刍兽考德里氏体（心水病病原体）对绵羊进行免疫接种，可诱导产生针对同源强毒细胞培养来源的反刍兽考德里氏体的保护性免疫。这种保护性免疫与高滴度的反刍兽考德里氏体特异性抗体的产生、脑内皮细胞中低水平的立克次体病以及无死亡病例相关。相比之下，未免疫的绵羊受到攻击时，脑内皮细胞中出现更高水平的立克次体病，其中一些死亡。这种针对心水病的免疫接种方法有可能取代目前使用的感染和治疗方法。
