被噬菌体PBS 1感染的枯草芽孢杆菌中脱氧核糖核酸酶活性的变化
Changes in DNase activities in Bacillus subtilis infected with bacteriophage PBS 1.
作者信息
Tomita F
出版信息
J Virol. 1975 May;15(5):1073-80. doi: 10.1128/JVI.15.5.1073-1080.1975.
Abstract
DNase activities in Bacillus subtilis were fractionated by chromatography on hydroxylapatite. One of the fractions hydrolyzed uracil-containing phage DNA but had no activity on host DNA. This activity on native phage DNA disappeared soon after phage infection, whereas DNase activities on bacterial DNA remained at the same level during phage development. An inhibitor of protein nature was induced by phage infection and this inhibitor was shown to be responsible for the disappearance of the DNase activity on phage DNA. Bacterial DNA in infected cells might be fragmented but is not degraded to acid-soluble oligonucleotides.
摘要
通过羟基磷灰石色谱法对枯草芽孢杆菌中的脱氧核糖核酸酶（DNase）活性进行了分级分离。其中一个级分能水解含尿嘧啶的噬菌体DNA，但对宿主DNA没有活性。这种对天然噬菌体DNA的活性在噬菌体感染后很快消失，而在噬菌体发育过程中，对细菌DNA的DNase活性保持在相同水平。噬菌体感染诱导了一种蛋白质性质的抑制剂，并且这种抑制剂被证明是导致噬菌体DNA上DNase活性消失的原因。受感染细胞中的细菌DNA可能会被片段化，但不会降解为酸溶性寡核苷酸。
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