含尿嘧啶DNA的代谢：枯草芽孢杆菌中噬菌体PBS2 DNA的降解
Metabolism of uracil-containing DNA: degradation of bacteriophage PBS2 DNA in Bacillus subtilis.
作者信息
Duncan B K, Warner H R
出版信息
J Virol. 1977 Jun;22(3):835-8. doi: 10.1128/JVI.22.3.835-838.1977.
Abstract
When Bacillus subtilis is infected by the uracil-containing DNA phage PBS2, the parental DNA labeled with radioactive uracil and cytosine remains acid insoluble. If the synthesis of the phage-induced uracil-DNA N-glycosidase inhibitor is prevented, the parental DNA is completely degraded to acid-soluble products beginning at about 6 min after infection. The host N-glycosidase probably initiates the degradation pathway, with nucleases being responsible for the remaining degradation of the DNA.
摘要
当枯草芽孢杆菌被含尿嘧啶的DNA噬菌体PBS2感染时，用放射性尿嘧啶和胞嘧啶标记的亲本DNA仍不溶于酸。如果噬菌体诱导的尿嘧啶-DNA N-糖苷酶抑制剂的合成受到抑制，亲本DNA在感染后约6分钟开始完全降解为酸溶性产物。宿主N-糖苷酶可能启动降解途径，核酸酶负责DNA的其余降解。
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8
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9
Bacillus subtilis deoxyuridinetriphosphatase and its bacteriophage PBS2-induced inhibitor.枯草芽孢杆菌脱氧尿苷三磷酸酶及其噬菌体PBS2诱导的抑制剂。
J Biol Chem. 1975 Nov 25;250(22):8804-11.
10
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J Virol. 1975 Aug;16(2):315-21. doi: 10.1128/JVI.16.2.315-321.1975.
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