来自拟杆菌菌株的两个5-硝基咪唑抗性决定簇以及两个基因上游一个新插入序列的核苷酸序列分析。
Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacteroides strains and of a new insertion sequence upstream of the two genes.
作者信息
Haggoud A, Reysset G, Azeddoug H, Sebald M
机构信息
Unité des Anaérobies, Institut Pasteur, Paris, France.
出版信息
Antimicrob Agents Chemother. 1994 May;38(5):1047-51. doi: 10.1128/AAC.38.5.1047.
Abstract
DNA sequence analysis of regions from plasmid pIP417 and chromosome BF8 which encode 5-nitroimidazole resistance in Bacteroides strains allowed the identification of two open reading frames corresponding to new genes, nimA (528 bp) and nimB (492 bp). Either gene may confer 5-nitroimidazole resistance to susceptible strains of Bacteroides. The encoded polypeptides have deduced molecular masses of 20.1 and 18.6 kDa, respectively, and share about 73% identity and 85% similarity. A new insertion sequence (IS) element named IS1168 lies 14 bases upstream of the nimA gene. The complete sequence of IS1168 was determined. A similar IS exists 12 bp upstream of the nimB gene. About 60% of the BF8 IS element was also sequenced and shown to be almost identical to IS1168.
摘要
对编码拟杆菌属菌株中5-硝基咪唑抗性的质粒pIP417和染色体BF8区域进行DNA序列分析，鉴定出两个与新基因相对应的开放阅读框，即nimA（528 bp）和nimB（492 bp）。任一基因均可使拟杆菌属敏感菌株产生5-硝基咪唑抗性。所编码的多肽推导分子量分别为20.1 kDa和18.6 kDa，序列一致性约为73%，相似性约为85%。一个名为IS1168的新插入序列元件位于nimA基因上游14个碱基处。测定了IS1168的完整序列。在nimB基因上游12 bp处存在一个相似的插入序列。还对BF8插入序列元件约60%的部分进行了测序，结果显示其与IS1168几乎相同。
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