Chochola J, Yamaguchi Y, Moguilevsky N, Bollen A, Strosberg A D, Stanislawski M
Laboratoire d'Immunologie, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Antimicrob Agents Chemother. 1994 May;38(5):969-72. doi: 10.1128/AAC.38.5.969.
Myeloperoxidase is virucidal to human immunodeficiency virus type 1 (HIV-1) in the persistently infected CEM human T-cell line or in acutely infected human peripheral blood mononuclear cells, as judged by viral infectivity and P24 radioimmunoassay. HIV-1 was specifically inactivated by low doses of the human myeloperoxidase (1.4 to 14.3 mU/ml) and the cells were spared. A higher enzyme concentration (143 mU/m) was cytotoxic, but uninfected CEM cells and normal lymphocytes were resistant to > or = 143 mU of myeloperoxidase per ml. The enzyme was virucidal with the Cl- present in medium and did not require exogenous H2O2. Catalase, an antioxidant enzyme, partially inhibited the virucidal effect of myeloperoxidase. Hence, the H2O2 probably came from the HIV-infected cells themselves. These in vitro findings indicate that the myeloperoxidase system is capable of inactivating HIV-1 of infected cells.
通过病毒感染性和P24放射免疫测定法判断，髓过氧化物酶对持续感染的CEM人T细胞系或急性感染的人外周血单核细胞中的1型人类免疫缺陷病毒（HIV-1）具有杀病毒作用。低剂量的人髓过氧化物酶（1.4至14.3 mU/ml）可特异性灭活HIV-1，且细胞未受影响。较高的酶浓度（143 mU/ml）具有细胞毒性，但未感染的CEM细胞和正常淋巴细胞对每毫升≥143 mU的髓过氧化物酶具有抗性。该酶在培养基中存在Cl-的情况下具有杀病毒作用，且不需要外源性H2O2。过氧化氢酶是一种抗氧化酶，可部分抑制髓过氧化物酶的杀病毒作用。因此，H2O2可能来自被HIV感染的细胞本身。这些体外研究结果表明，髓过氧化物酶系统能够灭活感染细胞中的HIV-1。
