Ogata Y, Yokota Y, Niisato N, Furuyama S, Sugiya H
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Arch Oral Biol. 1994 Jun;39(6):529-33. doi: 10.1016/0003-9969(94)90150-3.
When bovine cementum and bone were subjected to sequential dissociative extraction first with 4 M guanidine HCl ('G' extract) and then with 4 M guanidine HCl/0.5 M EDTA ('E' extract), high chemotactic activities for periodontal ligament cells were detected in the 'G' extracts using modified Boyden chambers. The chemotactic activities in both tissue extracts were destroyed by trypsin and heat. However, the activities in bone extracts were more heat-stable than those in cementum. When both tissue extracts were separated into bound and unbound fractions by DEAE-Sephacel ion-exchange chromatography, the chemotactic activities in cementum were detected in the unbound fractions, and those in bone in the bound fractions, which were eluted by 1 M NaCl. These results suggest that cementum and bone contain different chemotactic protein factors for periodontal ligament cells.
当牛牙骨质和骨组织先用4M盐酸胍（“G”提取物）进行顺序解离提取，然后再用4M盐酸胍/0.5M乙二胺四乙酸（“E”提取物）进行提取时，使用改良的博伊登小室在“G”提取物中检测到了对牙周膜细胞的高趋化活性。两种组织提取物中的趋化活性都被胰蛋白酶和加热破坏。然而，骨提取物中的活性比牙骨质中的活性更耐热。当两种组织提取物通过DEAE-葡聚糖凝胶离子交换色谱法分离为结合部分和未结合部分时，牙骨质中的趋化活性在未结合部分中检测到，而骨中的趋化活性在结合部分中检测到，结合部分用1M氯化钠洗脱。这些结果表明，牙骨质和骨含有不同的牙周膜细胞趋化蛋白因子。
