Metzger Z, Weinstock B, Dotan M, Narayanan A S, Pitaru S
Department of Oral Biology, Maurice and Gabriela Goldshleger School of Dental Medicine Tel Aviv University, Israel.
J Periodontal Res. 1998 Feb;33(2):126-9. doi: 10.1111/j.1600-0765.1998.tb02301.x.
Selective re-population of the root surface by periodontal ligament cells is considered a key factor in periodontal regeneration. A recently isolated cementum attachment protein (CAP) has been shown to enhance fibroblast attachment. In the present study the potential of CAP to selectively attract periodontal ligament cells (PLC) was studied in vitro in a micro-chemotaxis system. Human periodontal ligament cells and gingival fibroblasts (GF) were compared for their chemotactic response to either cementum attachment protein or to fibronectin. Murine dermal fibroblasts (MDF) served as control, irrelevant to the periodontium. The chemotactic response of PLC to fibronectin at 10(-8) M was of a similar magnitude as that of GF (16 +/- 5 and 11 +/- 3 cells/field, respectively), but both were significantly lower than the response of MDF (28 +/- 3 cells/field). The chemotactic response of periodontal ligament cells to the cementum attachment protein at 10(-7) M was higher (36 +/- 5 cells/field) than that of gingival fibroblasts or murine dermal fibroblasts (14 +/- 2 and 16 +/- 2 cells/field, respectively). These results suggest that cementum attachment protein can influence the selective re-population of root surfaces by periodontal ligament cells.
牙周膜细胞对根面的选择性再定植被认为是牙周再生的关键因素。最近分离出的一种牙骨质附着蛋白（CAP）已被证明能增强成纤维细胞的附着。在本研究中，利用微趋化系统在体外研究了CAP选择性吸引牙周膜细胞（PLC）的潜力。比较了人牙周膜细胞和牙龈成纤维细胞（GF）对牙骨质附着蛋白或纤连蛋白的趋化反应。小鼠皮肤成纤维细胞（MDF）作为对照，与牙周组织无关。PLC对10^(-8) M纤连蛋白的趋化反应与GF相似（分别为16±5和11±3个细胞/视野），但两者均显著低于MDF的反应（28±3个细胞/视野）。牙周膜细胞对10^(-7) M牙骨质附着蛋白的趋化反应高于牙龈成纤维细胞或小鼠皮肤成纤维细胞（分别为36±5和14±2以及16±2个细胞/视野）。这些结果表明，牙骨质附着蛋白可影响牙周膜细胞对根面的选择性再定植。
