Mariscal A, García A, Carnero M, Gómez E, Fernández-Crehuet J
Department of Preventive Medicine and Public Health, School of Medicine, University of Málaga, Spain.
Biotechniques. 1994 May;16(5):888-93.
We describe a new method that uses a fluorogenic bioassay of the beta-glucuronidase conversion of 4-methylumbelliferyl beta-D-glucuronide (MUG) to 4-methylumbelliferone to evaluate the individual toxic effects on Escherichia coli of Al3+, Cr6+, Hg2+ and Li+. This work was designed to examine the effectiveness of this method to measure the effects of five ionic concentrations of either Al3+, Cr6+, Hg2+ or Li+, on the growth of E. coli in a minimal medium that had MUG as the only source of carbon. This method was simple and fast, and its toxicity detection sensitivity was equal to, or greater than, existing bacterial bioassays. The use of the MUG substrate minimized the danger of interference by bacteria other than E. coli. Evaluations of toxicity in samples of public drinking water proved equally sensitive.
我们描述了一种新方法，该方法利用一种荧光生物测定法，即通过检测β-葡萄糖醛酸酶将4-甲基伞形酮基β-D-葡萄糖醛酸苷（MUG）转化为4-甲基伞形酮的过程，来评估Al3+、Cr6+、Hg2+和Li+对大肠杆菌的个体毒性作用。这项工作旨在检验该方法在以MUG作为唯一碳源的基本培养基中，测量五种离子浓度的Al3+、Cr6+、Hg2+或Li+对大肠杆菌生长影响的有效性。该方法简单快速，其毒性检测灵敏度与现有的细菌生物测定法相当或更高。使用MUG底物可将除大肠杆菌以外的其他细菌产生干扰的风险降至最低。对公共饮用水样本的毒性评估结果同样灵敏。
