Häggblom M M, Nohynek L J, Palleroni N J, Kronqvist K, Nurmiaho-Lassila E L, Salkinoja-Salonen M S, Klatte S, Kroppenstedt R M, Hägglblom M M
Center for Agricultural Molecular Biology, Rutgers University, New Brunswick, New Jersey 08903.
Int J Syst Bacteriol. 1994 Jul;44(3):485-93. doi: 10.1099/00207713-44-3-485.
Three independently isolated polychlorophenol-degrading strains of bacteria were characterized on the basis of chemotaxonomic and nutritional characteristics. Previously, these strains were assigned to the species Rhodococcus chlorophenolicus, which was described on the basis of the properties of one of the strains, strain PCP-I(T) (T = type strain) (J. H. A. Apajalahti, P. Kärpänoja, and M. S. Salkinoja-Salonen, Int. J. Syst. Bacteriol 36:246-251, 1986). However, the results of analyses of mycolic acids suggested that these organisms should be transferred to the genus Mycobacterium as Mycobacterium chlorophenolicum. These bacteria have meso-diaminopimelic acid, arabinose, and galactose as cell wall constituents, mycolic acids containing 75 to 80 carbon atoms, and a predominant menaquinone with nine isoprenoid units and one hydrogenated double bond. The fatty acids include mainly straight-chain saturated and monounsaturated fatty acids with 10 to 18 carbon atoms and a large proportion of 10-methyloctadecanoic acid (tuberculostearic acid). The G+C contents of the DNAs of the three strains range from 67 to 69 mol%.
基于化学分类学和营养特征对三株独立分离的多氯苯酚降解细菌菌株进行了特性鉴定。此前，这些菌株被归为氯酚红球菌，该菌种是根据其中一株菌株PCP-I(T)（T = 模式菌株）的特性描述的（J. H. A. Apajalahti、P. Kärpänoja和M. S. Salkinoja-Salonen，《国际系统细菌学杂志》36:246 - 251，1986年）。然而，分枝菌酸分析结果表明，这些微生物应作为氯酚分枝杆菌转移到分枝杆菌属。这些细菌的细胞壁成分含有内消旋二氨基庚二酸、阿拉伯糖和半乳糖，分枝菌酸含有75至80个碳原子，主要的甲基萘醌含有9个异戊二烯单元和一个氢化双键。脂肪酸主要包括含10至18个碳原子的直链饱和脂肪酸和单不饱和脂肪酸，以及大量的10-甲基十八烷酸（结核硬脂酸）。这三株菌株DNA的G+C含量范围为67至69 mol%。
