Briglia M, Rainey F A, Stackebrandt E, Schraa G, Salkinoja-Salonen M S
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
Int J Syst Bacteriol. 1996 Jan;46(1):23-30. doi: 10.1099/00207713-46-1-23.
A bacterial strain that was able to mineralize 2,4,6-trichlorophenol was isolated from a chlorophenol-fed percolator and was identified as a member of the genus Rhodococcus on the basis of chemotaxonomic characteristics and 16S RNA phylogenetic inference data. This organism (strain MBS1T [T = type strain]) exhibited a typical irregular rod-coccus cycle, and the cells had fimbria-like structures on their surfaces. The diagnostic cell wall amino acid was meso-diaminopimelic acid, and the sugars were arabinose and galactose; the mycolic acids contained 46 to 54 carbon atoms. The main menaquinone was MK-8(H2), and MK-9(H2) was a minor component. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylglycerol, and diphosphatidylglycerol. Tuberculostearic acid was present. The whole-cell fatty acids were straight-chain acids with 14 to 18 C atoms. The G+C content of the DNA was 67.4 mol%. This organism grew on sucrose, pyruvate, and 2,4,6-trichlorophenol, and it oxidized a large number of carbon compounds, including catechol, 3-hydroxyphenylacetic acid, and phenol. It also exhibited beta-galactosidase, urease, and 2-acetyl-lactate decarboxylase activities. On a phylogenetic tree that was based on 16S ribosomal DNA gene sequences strain MBS1T was found among the rhodococci on an independent branch. On the basis of the chemotaxonomic and phenotypic characteristics of strain MBS1T and its phylogenetic position we suggest that this bacterium should be placed in a new species, Rhodococcus percolatus; the specific epithet was chosen because the organism was isolated by using an enriched percolator. The type strain is strain MBS1.
从以氯酚为食的渗滤器中分离出一株能够使2,4,6 - 三氯苯酚矿化的细菌菌株，根据化学分类特征和16S RNA系统发育推断数据，该菌株被鉴定为红球菌属的一个成员。这种微生物（菌株MBS1T [T = 模式菌株]）表现出典型的不规则杆状 - 球菌循环，细胞表面有菌毛状结构。诊断性细胞壁氨基酸为内消旋二氨基庚二酸，糖类为阿拉伯糖和半乳糖；分枝菌酸含有46至54个碳原子。主要的甲基萘醌是MK - 8(H2)，MK - 9(H2)是次要成分。细胞磷脂为磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、磷脂酰甘油和二磷脂酰甘油。存在结核硬脂酸。全细胞脂肪酸为含有14至18个碳原子的直链酸。DNA的G + C含量为67.4 mol%。该微生物能在蔗糖、丙酮酸和2,4,6 - 三氯苯酚上生长，并且能氧化大量碳化合物，包括儿茶酚、3 - 羟基苯乙酸和苯酚。它还表现出β - 半乳糖苷酶、脲酶和2 - 乙酰乳酸脱羧酶活性。在基于16S核糖体DNA基因序列构建的系统发育树上，菌株MBS1T在红球菌中位于一个独立分支上。根据菌株MBS1T的化学分类和表型特征及其系统发育位置，我们建议将这种细菌归入一个新物种，即渗滤红球菌；选择这个种加词是因为该微生物是通过使用富集渗滤器分离得到的。模式菌株是MBS1菌株。
