通过脉冲场凝胶电泳、脂肪酸谱分型、血清分型和生物分型对肉类加工厂分离出的弯曲杆菌进行流行病学分型。
Epidemiological typing of Campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping.
作者信息
Steele M, McNab B, Fruhner L, DeGrandis S, Woodward D, Odumeru J A
机构信息
Laboratory Services Division, University of Guelph, Ontario, Canada.
出版信息
Appl Environ Microbiol. 1998 Jul;64(7):2346-9. doi: 10.1128/AEM.64.7.2346-2349.1998.
Abstract
Campylobacter spp. are a leading cause of bacterial gastroenteritis. Foods of animal origin, particularly under-cooked poultry, are common sources of Campylobacter species associated with disease in humans. A collection of 110 Campylobacter jejuni and 31 C. coli human and environmental isolates from different Ontario, Canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. Previously collected serotyping data for the same isolates were also analyzed in this study. Pulsed-field gel electrophoresis was found to be the most discriminatory of the typing methods, followed by serotyping, fatty acid profile typing, and biotyping. A wide variety of typing profiles were observed within the isolates, suggesting that several different Campylobacter sp. strains were present within the abattoirs.
摘要
弯曲杆菌属是细菌性肠胃炎的主要病因。动物源性食品，尤其是未煮熟的家禽，是与人类疾病相关的弯曲杆菌属的常见来源。对从加拿大安大略省不同屠宰场收集的110株空肠弯曲杆菌和31株大肠弯曲杆菌的人类及环境分离株进行了脉冲场凝胶电泳、脂肪酸谱分型和生物分型分析。本研究还分析了之前收集的相同分离株的血清分型数据。结果发现，脉冲场凝胶电泳是分型方法中鉴别力最强的，其次是血清分型、脂肪酸谱分型和生物分型。在分离株中观察到多种分型谱，这表明屠宰场内存在几种不同的弯曲杆菌菌株。
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