Steele M, McNab B, Fruhner L, DeGrandis S, Woodward D, Odumeru J A
Laboratory Services Division, University of Guelph, Ontario, Canada.
Appl Environ Microbiol. 1998 Jul;64(7):2346-9. doi: 10.1128/AEM.64.7.2346-2349.1998.
Campylobacter spp. are a leading cause of bacterial gastroenteritis. Foods of animal origin, particularly under-cooked poultry, are common sources of Campylobacter species associated with disease in humans. A collection of 110 Campylobacter jejuni and 31 C. coli human and environmental isolates from different Ontario, Canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. Previously collected serotyping data for the same isolates were also analyzed in this study. Pulsed-field gel electrophoresis was found to be the most discriminatory of the typing methods, followed by serotyping, fatty acid profile typing, and biotyping. A wide variety of typing profiles were observed within the isolates, suggesting that several different Campylobacter sp. strains were present within the abattoirs.
弯曲杆菌属是细菌性肠胃炎的主要病因。动物源性食品，尤其是未煮熟的家禽，是与人类疾病相关的弯曲杆菌属的常见来源。对从加拿大安大略省不同屠宰场收集的110株空肠弯曲杆菌和31株大肠弯曲杆菌的人类及环境分离株进行了脉冲场凝胶电泳、脂肪酸谱分型和生物分型分析。本研究还分析了之前收集的相同分离株的血清分型数据。结果发现，脉冲场凝胶电泳是分型方法中鉴别力最强的，其次是血清分型、脂肪酸谱分型和生物分型。在分离株中观察到多种分型谱，这表明屠宰场内存在几种不同的弯曲杆菌菌株。
