Küchler R
Zentralbl Bakteriol Orig A. 1975;230(2):192-202.
A further differentiation of strains of Pseudomonas aeruginosa which have been isolated from clinical material is desirable because of the increasing frequency of hospital infections caused by this species. Only by such a procedure cross infections which require hygienic measures can be distinguished from autoinfections. Pyocine typing with a set of only four indicator strains was tried by repeated typing of 542 strains. Because of its simplicity and the small amount of material and working time required the method was found to be suitable for routine application. Reproducibility was satisfactory. The strains tested could be grouped into nine different pyocine types. Similar to the results of other typing procedures, 74 per cent of the strains belonged to three groups. The method is suitable for detection of sources of hospital infections caused by the more rare pyocine types. By a comparison of the antibiotic resistance patterns with pyocine types in strains which had been isolated from patients with chronic infections it was demonstrated that antibiotic resistance patterns could not be used as criteria for epidemiological investigations.
鉴于铜绿假单胞菌引起医院感染的频率不断增加，对从临床材料中分离出的该菌菌株进行进一步区分是很有必要的。只有通过这种方法，才能将需要采取卫生措施的交叉感染与自身感染区分开来。我们通过对542株菌株进行重复分型，尝试使用仅一组四种指示菌株进行绿脓菌素分型。由于该方法简单，所需材料和工作时间少，因此被认为适用于常规应用。重复性令人满意。所测试的菌株可分为九种不同的绿脓菌素类型。与其他分型方法的结果相似，74%的菌株属于三个组。该方法适用于检测由较罕见的绿脓菌素类型引起的医院感染源。通过比较从慢性感染患者分离出的菌株的抗生素耐药模式和绿脓菌素类型，结果表明抗生素耐药模式不能用作流行病学调查的标准。
