核苷酸序列变化对大肠杆菌中DNA二级结构形成的影响与体内十字形突出一致。
The effects of nucleotide sequence changes on DNA secondary structure formation in Escherichia coli are consistent with cruciform extrusion in vivo.
作者信息
Davison A, Leach D R
机构信息
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
出版信息
Genetics. 1994 Jun;137(2):361-8. doi: 10.1093/genetics/137.2.361.
Abstract
The construction in bacteriophage lambda of a set of long DNA palindromes with paired changes in the central sequence is described. Identical palindrome centers were previously used by others to test the S-type model for cruciform extrusion in vitro. Long DNA palindromes prevent the propagation of carrier phage lambda on a wild-type host, and the sbcC mutation is sufficient to almost fully alleviate this inviability. The plaque areas produced by the palindrome containing phages were compared on an Escherichia coli sbcC lawn. Central sequence changes had a greater effect upon the plaque area than peripheral changes, implying that the residual palindrome-mediated inviability in E. coli sbcC is center-dependent and could be due to the formation of a cruciform structure. The results argue strongly that intrastrand pairing within palindromes is critical in determining their effects in vivo. In addition, the same data suggests that DNA loops in vivo may sometimes contain two bases only.
摘要
本文描述了在噬菌体λ中构建一组具有中央序列配对变化的长DNA回文序列。之前其他人曾使用相同的回文序列中心在体外测试十字形挤出的S型模型。长DNA回文序列会阻止携带噬菌体λ在野生型宿主上繁殖，而sbcC突变足以几乎完全缓解这种致死性。在大肠杆菌sbcC菌苔上比较了含回文序列的噬菌体产生的噬菌斑面积。中央序列变化对噬菌斑面积的影响大于周边变化，这意味着大肠杆菌sbcC中残留的回文序列介导的致死性是中心依赖性的，可能是由于十字形结构的形成。结果有力地表明，回文序列内的链内配对对于确定其在体内的作用至关重要。此外，相同的数据表明体内的DNA环有时可能仅包含两个碱基。
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本文引用的文献
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Cruciform formation in a negatively supercoiled DNA may be kinetically forbidden under physiological conditions.在生理条件下，负超螺旋DNA中的十字形结构形成可能在动力学上受到抑制。
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