与显性标记进行杂交时重组频率估计的稳健性。
The robustness of recombination frequency estimates in intercrosses with dominant markers.
作者信息
Säll T, Nilsson N O
机构信息
Department of Genetics, Lund University, Sweden.
出版信息
Genetics. 1994 Jun;137(2):589-96. doi: 10.1093/genetics/137.2.589.
The robustness of the maximum likelihood estimates of recombination frequencies has been investigated in double intercrosses with complete dominance at both loci. The robustness was investigated with respect to bias in the recombination frequency estimates due to: (1) limited sample sizes, (2) heterogeneity in recombination frequencies between sexes or among meioses and (3) factors that distort the segregation-misclassification or differential viability. In the coupling phase, the recombination frequency estimates are quite robust with respect to most of the investigated factors. Potentially, the most serious cause of a bias is misclassifications, which tend to increase the recombination frequency estimates. In the repulsion phase, misclassifications are particularly serious, leading to extreme discrepancies between true and observed values. In addition, limited sample size and sex differences in recombination can also bias recombination frequency estimates in repulsion. These effects may pose serious problem in genetic mapping with random amplified polymorphic DNA (RAPD) markers.
在两个位点均为完全显性的双杂交中，研究了重组频率最大似然估计值的稳健性。针对因以下原因导致的重组频率估计偏差，对稳健性进行了研究：（1）样本量有限；（2）性别间或减数分裂间重组频率的异质性；（3）扭曲分离错误分类或差异生存能力的因素。在相引相中，重组频率估计值对于大多数研究因素相当稳健。潜在地，偏差最严重的原因是错误分类，这往往会增加重组频率估计值。在相斥相中，错误分类尤为严重，导致真实值与观测值之间出现极大差异。此外，样本量有限以及重组中的性别差异也会使相斥相中的重组频率估计产生偏差。这些效应可能会给随机扩增多态性DNA（RAPD）标记的基因定位带来严重问题。
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