抗粒细胞抗体IgG的化学发光免疫测定法

[Chemiluminescent immunoassay for anti-granulocyte antibody IgG].

作者信息

Peng D, Luo X, Wang J, Yang X, Fan Z

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1994 Mar;25(1):108-11.

Abstract

In this paper is reported solid phase competitive chemiluminescent immunoassay (CLIA) for anti-granulocyte antibody (AGAb) IgG with N-(4-amiobytul)-N-ethylisoluminol (ABEI) labelled rabbit antibody IgG against granulocyte competing with the analyte and ABEI-CoCl2-H2O2 as the chemiluminescent system. The absolute limit of detection was 3.0 x 10(-14) mol ABEI/tube. One hundred and thirty one individuals were divided into three groups: leukocytopenic group (n = 58), control group (n = 41) and normal group (n = 32). Chemiluminescent index (CLI) was used as the diagnostic criterion for AGAb in serum. The results showed that the positive rate of the leukocytopenic group was significantly higher than that of the control group (P < 0.005), and the sensitivity, specificity and accuracy of the method were 43.1%, 90.2% and 62.6%, respectively.

摘要

本文报道了一种固相竞争化学发光免疫分析法（CLIA），用于检测抗粒细胞抗体（AGAb）IgG。该方法采用N-(4-氨基丁基)-N-乙基异鲁米诺（ABEI）标记的抗粒细胞兔抗体IgG与分析物竞争，以ABEI-CoCl₂-H₂O₂作为化学发光体系。检测的绝对限为3.0×10⁻¹⁴ mol ABEI/管。131名个体分为三组：白细胞减少组（n = 58）、对照组（n = 41）和正常组（n = 32）。采用化学发光指数（CLI）作为血清中AGAb的诊断标准。结果显示，白细胞减少组的阳性率显著高于对照组（P < 0.005），该方法的灵敏度、特异性和准确性分别为43.1%、90.2%和62.6%。