LysR家族调控因子ClcR的纯化及其与恶臭假单胞菌clcABD氯儿茶酚操纵子启动子的相互作用。
Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.
作者信息
Coco W M, Parsek M R, Chakrabarty A M
机构信息
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60612.
出版信息
J Bacteriol. 1994 Sep;176(17):5530-3. doi: 10.1128/jb.176.17.5530-5533.1994.
Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR. In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.
先前的研究表明，clcABD操纵子受LysR型激活剂ClcR的转录控制。在本研究中，避免了导致其聚集的条件，并对ClcR进行了纯化，通过氨基末端测序进行了确认。凝胶过滤表明，ClcR在溶液中以二聚体形式存在。测定了ClcR的DNase I足迹。比较了ClcR与儿茶酚操纵子调节因子CatR的结合特性。
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