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破伤风毒素通过影响突触小泡结合蛋白突触囊泡蛋白，阻断了鱿鱼巨突触的神经传递。

Transmission at the squid giant synapse was blocked by tetanus toxin by affecting synaptobrevin, a vesicle-bound protein.

作者信息

Llinás R, Sugimori M, Chu D, Morita M, Blasi J, Herreros J, Jahn R, Marsal J

机构信息

Department of Physiology and Biophysics, New York University Medical Center, NY 10016.

出版信息

J Physiol. 1994 May 15;477(Pt 1):129-33. doi: 10.1113/jphysiol.1994.sp020177.

DOI:10.1113/jphysiol.1994.sp020177
PMID:8071879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1155580/
Abstract
  1. The effect of whole tetanus toxin (TeTX) and of its light chain (TeTX L-chain) on transmitter release was determined by presynaptic pressure-injection in the squid giant synapse. 2. The results indicate that whole TeTX does not modify transmission while the L-chain blocks transmission within 20-30 min. This block does not involve changes in the sodium or potassium conductances responsible for spike generation or the voltage-dependent presynaptic calcium current responsible for transmitter release. 3. Western blotting of protein fractions from the squid optic lobe demonstrated the presence of a protein which reacted with specific antibodies against mammalian synaptobrevin, a vesicular protein. In addition, this protein was enzymatically cleaved by the L-chain component of the toxin in a similar fashion to its mammalian counterpart. 4. These results demonstrate that TeTX L-chain toxin acts directly on a squid synaptobrevin and prevents synaptic release probably by interfering with the docking-fusion synaptic vesicles at the active zone.
摘要
  1. 通过在枪乌贼巨大突触中进行突触前压力注射，确定了全破伤风毒素（TeTX）及其轻链（TeTX L链）对递质释放的影响。2. 结果表明，全TeTX不改变传递，而L链在20 - 30分钟内阻断传递。这种阻断不涉及负责动作电位产生的钠或钾电导的变化，也不涉及负责递质释放的电压依赖性突触前钙电流的变化。3. 对枪乌贼视叶蛋白质组分进行的蛋白质印迹分析表明，存在一种与抗哺乳动物突触囊泡蛋白突触小泡蛋白的特异性抗体发生反应的蛋白质。此外，该蛋白质被毒素的L链成分酶切，其方式与其哺乳动物对应物相似。4. 这些结果表明，TeTX L链毒素直接作用于枪乌贼的突触小泡蛋白，并可能通过干扰突触小泡在活性区的对接融合来阻止突触释放。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c7/1155580/5e80cb8c9b33/jphysiol00348-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c7/1155580/5e80cb8c9b33/jphysiol00348-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c7/1155580/5e80cb8c9b33/jphysiol00348-0131-a.jpg

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