在α-1肾上腺素能受体刺激期间，通过31P-核磁共振测量分离的大鼠心脏中的细胞内pH值。
Intracellular pH measured by 31P-NMR in isolated rat heart during alpha-1-adrenoceptor stimulation.
作者信息
Hanem S, Grønås T, Holten T, Jynge P, Skomedal T, Osnes J B
机构信息
Department of Pharmacology, University of Oslo, Norway.
出版信息
J Mol Cell Cardiol. 1994 May;26(5):669-74. doi: 10.1006/jmcc.1994.1079.
Changes of intracellular pH (pHi) after alpha-1-adrenoceptor stimulation (5 x 10(-5) mol/l phenylephrine in the presence of 10(-6) mol/l timolol) were studied in isolated perfused rat hearts by 31P-NMR spectroscopy in order to use the whole organ without disruption. The contractility was monitored simultaneously in all hearts. Alpha-1-adrenoceptor stimulation gave an alkalinization by 0.02 +/- 0.01 pH-units (mean +/- S.E.M.) after 5 min and by 0.03 +/- 0.01 pH-units (mean +/- S.E.M.) after 15 min. Thus, an intracellular alkalinization after myocardial alpha-1-adrenoceptor stimulation was observed during close to physiological conditions. The elevation of pHi may contribute to maintain the inotropic effect after alpha-1-adrenoceptor stimulation.
为了在不破坏整个器官的情况下进行研究，通过31P-核磁共振波谱法在离体灌注大鼠心脏中研究了α-1肾上腺素能受体刺激（在存在10(-6) mol/L噻吗洛尔的情况下，使用5×10(-5) mol/L去氧肾上腺素）后细胞内pH值（pHi）的变化。同时监测所有心脏的收缩力。α-1肾上腺素能受体刺激在5分钟后使pH值升高0.02±0.01个pH单位（平均值±标准误），在15分钟后升高0.03±0.01个pH单位（平均值±标准误）。因此，在接近生理条件下观察到心肌α-1肾上腺素能受体刺激后细胞内碱化。pHi的升高可能有助于维持α-1肾上腺素能受体刺激后的正性肌力作用。
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