Sitozhevskiĭ A V, Lusta I V, Trofimov A V, Ivanov V V, Karpenko O A
Probl Endokrinol (Mosk). 1994 May-Jun;40(3):39-41.
Rates of basal and glucose-stimulated insulin and glutathione secretion were studied in experiments with isolated rat pancreas, as were prooxidant effects on these values. The rate of oxidized and recovered glutathione release was found increased at glucose concentration increase to 16.7 mmoles in perfusion solution. Addition of prooxidants (tert-butyl hydroperoxide and Fe2+) in concentrations 10(-4) mole did not change basal insulin secretion but resulted in reduction of glucose-stimulated hormone release. Under such conditions a reduction of the rate of oxidized and recovered glutathione release by the pancreas was observed which was adequate to changed GSH/GSSG ratio in isolated Langerhans' islets. It may be supposed that lipid peroxidation results in changed thiol-disulfide ratio in Langerhans' islets B cells and in reduction of their sensitivity to secretogen effect.
在离体大鼠胰腺实验中，研究了基础及葡萄糖刺激的胰岛素和谷胱甘肽分泌速率，以及促氧化剂对这些数值的影响。发现当灌注液中葡萄糖浓度增加至16.7毫摩尔时，氧化型和还原型谷胱甘肽释放速率增加。添加浓度为10(-4)摩尔的促氧化剂（叔丁基过氧化氢和Fe2+）不会改变基础胰岛素分泌，但会导致葡萄糖刺激的激素释放减少。在这种情况下，观察到胰腺氧化型和还原型谷胱甘肽释放速率降低，这与分离的胰岛中谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值的变化相适应。可以推测，脂质过氧化导致胰岛B细胞中硫醇-二硫键比值改变，并降低其对促分泌剂作用的敏感性。
