Turkina T I, Marchenko L F, Sapelkina L V, Pozniak T I, Kiseleva E V
Probl Endokrinol (Mosk). 1994 May-Jun;40(3):6-8.
Children suffering from insulin-dependent diabetes mellitus (IDDM) were examined for unsaturation, that is, total quantity of double bonds in individual fractions of blood serum lipids was assessed. Lipid fractions were isolated by thin-layer chromatography. Unsaturation was assessed by ozonation method. The research was aimed at elucidation of biochemical criteria of the degree of metabolic disorders in children with IDDM and at development of methods for quantitative assessment of such disorders. Thirty-two inpatients with IDDM aged 7 to 15 were examined over time. Control group included 10 healthy age-matched children. Twenty children were examined during compensation stage (group 1), twelve during decompensation with ketoacidosis (group 2). Total unsaturation parameters were found reliably decreased in children with IDDM both during compensation and decompensation stages vs. those in healthy controls. A relative decrease of unsaturated fatty acids in the fraction of unesterified fatty acids (p < 0.05) was characteristic of group 2, with the content of their fragments in triglyceride fraction being the maximal (p < 0.05). The pattern of total unsaturation distribution in serum lipid fractions may serve as an indicator for metabolic disorders and decompensation in IDDM.
对患有胰岛素依赖型糖尿病（IDDM）的儿童进行了不饱和度检查，即评估血清脂质各组分中双键的总量。脂质组分通过薄层色谱法分离。不饱和度通过臭氧化法评估。该研究旨在阐明IDDM儿童代谢紊乱程度的生化标准，并开发定量评估此类紊乱的方法。对32名年龄在7至15岁的IDDM住院患者进行了长期检查。对照组包括10名年龄匹配的健康儿童。20名儿童在代偿期接受检查（第1组），12名在酮症酸中毒失代偿期接受检查（第2组）。与健康对照组相比，IDDM儿童在代偿期和失代偿期的总不饱和度参数均显著降低。第2组的特征是未酯化脂肪酸组分中不饱和脂肪酸相对减少（p<0.05），其片段在甘油三酯组分中的含量最高（p<0.05）。血清脂质组分中总不饱和度分布模式可作为IDDM代谢紊乱和失代偿的指标。
