Pegado M G, de Souza W
Laboratório de Ultraesturura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Parasitol Res. 1994;80(4):320-6. doi: 10.1007/BF02351874.
Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment of the parasites to the epithelial cells. Experiments in which the parasites or epithelial cells were treated before interaction showed that these monosaccharides interfered with both cell surfaces. Trypsin-sensitive, but not neuraminidase-sensitive, groups exposed on the cell surface are important for the parasite-epithelial cell association. Fluorescein isothiocyanate (FITC)- or colloidal gold-labeled lectins were used to analyse the distribution of carbohydrates on the surface of G. duodenalis and epithelial cells. It is important to stress here the presence of fucose on the parasite surface. Treatment of the cells with lectins was also used to analyse the role of carbohydrate-containing macromolecules in the parasite-cell interaction.
使用单糖、凝集素、高碘酸盐、胰蛋白酶和神经氨酸酶来分析十二指肠贾第虫滋养体与肠上皮细胞系IEC细胞的黏附过程。在相互作用培养基中添加N-乙酰葡糖胺、N-乙酰半乳糖胺、半乳糖和岩藻糖可抑制寄生虫与上皮细胞的附着。在相互作用前对寄生虫或上皮细胞进行处理的实验表明，这些单糖会干扰两者的细胞表面。细胞表面暴露的对胰蛋白酶敏感而非对神经氨酸酶敏感的基团对于寄生虫与上皮细胞的结合很重要。使用异硫氰酸荧光素（FITC）或胶体金标记的凝集素来分析十二指肠贾第虫和上皮细胞表面碳水化合物的分布。在此需要强调的是寄生虫表面存在岩藻糖。用凝集素处理细胞也用于分析含碳水化合物的大分子在寄生虫与细胞相互作用中的作用。
