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Isolation and partial characterization of crystal matrix protein as a potent inhibitor of calcium oxalate crystal aggregation: evidence of activation peptide of human prothrombin.

作者信息

Suzuki K, Moriyama M, Nakajima C, Kawamura K, Miyazawa K, Tsugawa R, Kikuchi N, Nagata K

机构信息

Department of Urology, Kanazawa Medical University, Uchinada, Japan.

出版信息

Urol Res. 1994;22(1):45-50. doi: 10.1007/BF00431548.

DOI:10.1007/BF00431548
PMID:8073540
Abstract

In order to clarify the characteristics of crystal matrix protein (CMP), which exhibits a remarkable affinity for calcium oxalate crystals and may be important in stone pathogenesis, we have isolated CMP from macromolecular matrix substances of newly-formed calcium oxalate crystals. Purification of CMP consisted of calcium oxalate crystal formation, dissolution of crystals, electrodialysis, anion exchange chromatography and high-performance liquid chromatography. CMP showed the protein band of 31 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CMP was identical to that of human prothrombin. Both anti-CMP polyclonal antibody and anti-human prothrombin antibody cross-reacted well with human prothrombin and CMP in Western blotting. Its amino acid composition and its molecular weight of 31 kDa strongly suggest that CMP is the activation peptide of human prothrombin.

摘要

相似文献

1
Isolation and partial characterization of crystal matrix protein as a potent inhibitor of calcium oxalate crystal aggregation: evidence of activation peptide of human prothrombin.
Urol Res. 1994;22(1):45-50. doi: 10.1007/BF00431548.
2
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3
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4
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本文引用的文献

1
Purification and characterization of the principal inhibitor of calcium oxalate monohydrate crystal growth in human urine.人尿中一水草酸钙晶体生长主要抑制剂的纯化与特性研究
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2
Characterization of the complementary deoxyribonucleic acid and gene coding for human prothrombin.人凝血酶原互补脱氧核糖核酸及编码基因的特性分析
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The effect of urine, pyrophosphate, citrate, magnesium and glycosaminoglycans on the growth and aggregation of calcium oxalate crystals in vitro.
肾结石：肾结石形成的分子机制和调节剂的关键作用。
Biomed Res Int. 2013;2013:292953. doi: 10.1155/2013/292953. Epub 2013 Sep 14.
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Association between polymorphisms in osteopontin gene (SPP1) and first episode calcium oxalate urolithiasis.骨桥蛋白基因（SPP1）多态性与首次草酸钙尿石症发作的关系。
Urolithiasis. 2013 Aug;41(4):303-13. doi: 10.1007/s00240-013-0582-7. Epub 2013 Jun 20.
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Effects of oxalate exposure on Madin-Darby canine kidney cells in culture: renal prothrombin fragment-1 mRNA expression.草酸盐暴露对培养的Madin-Darby犬肾细胞的影响：肾凝血酶原片段-1信使核糖核酸表达
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In vitro crystallisation systems for the study of urinary stone formation.用于研究尿路结石形成的体外结晶系统。
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Association of urinary macromolecules with calcium oxalate crystals induced in vitro in normal human and rat urine.正常人尿液和大鼠尿液中体外诱导产生的尿大分子与草酸钙晶体的关联。
Urol Res. 1996;24(1):45-50. doi: 10.1007/BF00296733.
尿液、焦磷酸盐、柠檬酸盐、镁及糖胺聚糖对草酸钙晶体体外生长和聚集的影响。
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New method for quantitative determination of uronic acids.糖醛酸定量测定的新方法。
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5
Urine glycoprotein crystal growth inhibitors. Evidence for a molecular abnormality in calcium oxalate nephrolithiasis.尿糖蛋白晶体生长抑制剂。草酸钙肾结石分子异常的证据。
J Clin Invest. 1985 Oct;76(4):1455-62. doi: 10.1172/JCI112124.
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Macromolecules inhibit calcium oxalate crystal growth and aggregation in whole human urine.大分子抑制草酸钙晶体在全人尿液中的生长和聚集。
Clin Chim Acta. 1987 Aug 31;167(3):329-38. doi: 10.1016/0009-8981(87)90353-6.
7
Isolation from human calcium oxalate renal stones of nephrocalcin, a glycoprotein inhibitor of calcium oxalate crystal growth. Evidence that nephrocalcin from patients with calcium oxalate nephrolithiasis is deficient in gamma-carboxyglutamic acid.从人草酸钙肾结石中分离出肾钙蛋白，一种草酸钙晶体生长的糖蛋白抑制剂。草酸钙肾结石患者的肾钙蛋白缺乏γ-羧基谷氨酸的证据。
J Clin Invest. 1987 Jun;79(6):1782-7. doi: 10.1172/JCI113019.
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A new approach to the study of urinary macromolecules as a participant in calcium oxalate crystallization.一种将尿液大分子作为草酸钙结晶参与者进行研究的新方法。
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The influence of urinary macromolecules on calcium oxalate monohydrate crystal growth.尿大分子对一水合草酸钙晶体生长的影响。
J Urol. 1988 Jan;139(1):190-5. doi: 10.1016/s0022-5347(17)42351-2.
10
Crystal-matrix relationships in experimentally induced urinary calcium oxalate monohydrate crystals, an ultrastructural study.实验诱导的一水合草酸钙尿结石晶体中的晶体-基质关系：一项超微结构研究
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