Lanzalaco A C, Singh R P, Smesko S A, Nancollas G H, Sufrin G, Binette M, Binette J P
Department of Chemistry, State University of New York at Buffalo 14214.
J Urol. 1988 Jan;139(1):190-5. doi: 10.1016/s0022-5347(17)42351-2.
The Constant Composition (CC) kinetics method has been used for studying the mineralization of calcium oxalate monohydrate (COM) at sustained supersaturations in the presence of pre-bladder urine and macromolecules isolated from normal urine and kidney and bladder stones. The method is especially sensitive for investigating the inhibitory activities of these urinary macromolecular components (UMMC) and matrix macromolecular components (MMMC) with a coefficient of variation in growth rate of approximately 2%. Significant COM mineral inhibition was observed in a wide molecular weight region of urine components. Urine removed directly from the kidney showed appreciable inhibitory activity towards COM crystallization. Normal urinary proteins and the dissolved precipitate resulting from urine centrifugation were fractionated by gel filtration. The resulting solutions were mostly COM mineralization inhibitors. Electrodialysis was utilized to isolate the MMMC (greater than 7000 d) of renal and bladder calculi. While these solutions inhibited COM crystallization, they were also found to be calcium binders as measured by the calcium electrode.
恒组成（CC）动力学方法已被用于研究一水合草酸钙（COM）在持续过饱和状态下，于膀胱前尿液以及从正常尿液、肾和膀胱结石中分离出的大分子存在时的矿化情况。该方法对于研究这些尿大分子成分（UMMC）和基质大分子成分（MMC）的抑制活性特别灵敏，生长速率的变异系数约为2%。在尿成分的较宽分子量范围内观察到显著的COM矿化抑制作用。直接从肾脏取出的尿液对COM结晶显示出明显的抑制活性。通过凝胶过滤对直接从肾脏取出的尿液中的正常尿蛋白和尿液离心产生的溶解沉淀物进行分级分离。所得溶液大多是COM矿化抑制剂。利用电渗析分离肾和膀胱结石的MMC（大于7000 d）。虽然这些溶液抑制COM结晶，但通过钙电极测量发现它们也是钙结合剂。
