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Baculovirus expression of the nucleocapsid protein of a Puumala serotype Hantavirus.

作者信息

Schuldt C, Zöller L, Bautz E K, Darai G

机构信息

Institut für Molekulare Genetik der Universität Heidelberg, FRG.

出版信息

Virus Genes. 1994 Mar;8(2):143-9. doi: 10.1007/BF01703071.

DOI:10.1007/BF01703071
PMID:8073635
Abstract

Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18-20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HFRS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.

摘要

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本文引用的文献

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A novel mu-capture enzyme-linked immunosorbent assay based on recombinant proteins for sensitive and specific diagnosis of hemorrhagic fever with renal syndrome.一种基于重组蛋白的新型μ捕获酶联免疫吸附测定法，用于肾综合征出血热的灵敏且特异诊断。
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