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表达汉坦病毒核衣壳蛋白的重组杆状病毒作为诊断抗原在间接免疫荧光试验中的应用：引起肾综合征出血热（HFRS）的3种汉坦病毒血清型之间的交叉反应性。

Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS).

作者信息

Yoshimatsu K, Arikawa J, Kariwa H

机构信息

Institute of Immunological Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

J Vet Med Sci. 1993 Dec;55(6):1047-50. doi: 10.1292/jvms.55.1047.

DOI:10.1292/jvms.55.1047
PMID:7906956
Abstract

Recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (NP) of Seoul type hantavirus, strain SR-11 (rNP-SR-Sf9) were applied to the indirect immunofluorescent antibody (IFA) test. The rNP-SR-Sf9 reacted with anti NP MAb clones which recognized strain specific or hantavirus common epitopes in the IFA test. The recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (Hantaan 76-118, SR-11, Puumala). Antibody titers of a group of experimentally infected mouse and urban rat sera using rNP-SR-Sf9 and strain SR-11-infected Vero cell antigens were mutual correlative. These results indicated that the rNP-SR-Sf9 were an effective and safety substitute for Vero E6 cells in the IFA test for serosurveys of hantavirus infection.

摘要

表达重组蛋白的重组杆状病毒感染昆虫细胞，该重组蛋白类似于汉城型汉坦病毒SR - 11株的核衣壳蛋白（NP）（rNP - SR - Sf9），被应用于间接免疫荧光抗体（IFA）检测。在IFA检测中，rNP - SR - Sf9与识别毒株特异性或汉坦病毒共同表位的抗NP单克隆抗体克隆发生反应。该重组抗原可被3种汉坦病毒血清型（汉滩病毒76 - 118、SR - 11、普马拉病毒）的抗体检测到。使用rNP - SR - Sf9和SR - 11毒株感染的Vero细胞抗原，一组经实验感染的小鼠和城市大鼠血清的抗体滴度呈相互关联。这些结果表明，在汉坦病毒感染血清学调查的IFA检测中，rNP - SR - Sf9是Vero E6细胞的有效且安全的替代物。

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