Zöller L G, Yang S, Gött P, Bautz E K, Darai G
Ernst Rodenwaldt Institut, Fachbereich Medizinische Mikrobiologie, Koblenz, Germany.
J Clin Microbiol. 1993 May;31(5):1194-9. doi: 10.1128/jcm.31.5.1194-1199.1993.
Hantavirus nucleocapsid protein has recently been identified as a major antigen inducing an early and long-lasting humoral immune response in patients with hemorrhagic fever with renal syndrome. A mu-capture enzyme-linked immunosorbent assay utilizing recombinant nucleocapsid proteins of Hantavirus strains Hantaan 76-118 (Hantaan serotype) and CG 18-20 (Puumala serotype) as diagnostic antigens and specific monoclonal antibodies as the detection system has been developed. Histidine-tailed recombinant proteins were expressed in Escherichia coli and purified in a single step by affinity chromatography on a nickel-chelate resin. The assay was evaluated with a panel of sera from patients with hemorrhagic fever with renal syndrome originating from various geographic regions. The overall sensitivity of the mu-capture enzyme-linked immunosorbent assay (both recombinant antigens) was 100%, and its specificity was also found to be 100%. Immunoglobulin M antibodies were detected as early as on day 3, and maximum titers were obtained between days 8 and 25 after onset of the disease. The assay was regularly found to be positive within 3 to 4 months but in some cases up to 2 years after the acute phase of the disease.
汉坦病毒核衣壳蛋白最近被确定为肾综合征出血热患者中诱导早期和持久体液免疫反应的主要抗原。利用汉坦病毒株汉滩76 - 118（汉滩血清型）和CG 18 - 20（普马拉血清型）的重组核衣壳蛋白作为诊断抗原以及特异性单克隆抗体作为检测系统，开发了一种μ捕获酶联免疫吸附测定法。带有组氨酸尾的重组蛋白在大肠杆菌中表达，并通过镍螯合树脂上的亲和色谱一步纯化。该测定法用来自不同地理区域的肾综合征出血热患者的一组血清进行了评估。μ捕获酶联免疫吸附测定法（两种重组抗原）的总体敏感性为100%，其特异性也为100%。免疫球蛋白M抗体最早在第3天被检测到，发病后第8天至25天达到最高滴度。该测定法在疾病急性期后的3至4个月内经常呈阳性，但在某些情况下在疾病急性期后长达2年仍呈阳性。
