Kodama M, Kodama T
Kodama Research Institute of Preventive Medicine, Nagoya, Japan.
Anticancer Res. 1994 May-Jun;14(3A):1049-57.
The incidence of cervical cancer is known to decrease with the Westernization of life style. The purpose of this study was to see whether or not the practice of pseudopregnancy conditioning (psp-c) and/or non-Western (rice-rich or rice-and salt-rich) diet conditioning (dt-c) in female mice can reproduce a specific steroidal disorder that is associated with cervical cancer--general depression of androgen and partial depression of corticoid in urine. The effects of psp-c and dt-c were assessed by estimating various steroids in urine and plasma of mice, as collected at the terminal stage of the experiment. Macroscopic and microscopic changes of genital organs were also investigated by dissection. Results obtained are as follows: 1) the practice of psp-c and/or dt-c induced a total of 13 deciduomas in the vagina, the cervix and the corpus of experimental mice. No deciduoma was found in virgin mice fed a standard (Western-style) diet. 2) The combination of 2 reproductive markers (psp-c and non-psp-c) and 3 dietary markers (standard diet, rice-rich diet and rice- and salt-rich diet) produced a variety of changes in the excretions of all androgens, progestins and corticoids in the urine of mice for each of 5 experimental groups. A common steroidal trait was extracted from the urinary steroid data of 5 experimental groups--general depression of androgens, progestins and majority corticoids relative to tetrahydrocortisol. By the collation test, the combination of psp-c and rice- and salt-rich diet conditioning was found to be the best choice for reproducing the urinary steroid changes specific for cervical cancer. 3) The results of plasma steroid analysis, though not incompatible with the urinary steroid data, were fragmentary and not very informative in this study. The observed resistance of mice to carcinogenic insults together with the possible role of deciduoma in cervical carcinogenesis is discussed in the light of relevant information including the anti-carcinogenic action of vitamin C.
已知宫颈癌的发病率会随着生活方式的西化而降低。本研究的目的是观察对雌性小鼠进行假孕预处理（psp-c）和/或非西方（富含大米或富含大米和盐）饮食预处理（dt-c）是否能重现与宫颈癌相关的特定甾体紊乱——尿液中雄激素普遍降低以及皮质激素部分降低。通过在实验末期收集小鼠尿液和血浆中的各种甾体来评估psp-c和dt-c的效果。还通过解剖研究生殖器官的宏观和微观变化。得到的结果如下：1）psp-c和/或dt-c的处理在实验小鼠的阴道、子宫颈和子宫体共诱发了13个蜕膜瘤。在喂食标准（西式）饮食的处女小鼠中未发现蜕膜瘤。2）2种生殖标记（psp-c和非psp-c）与3种饮食标记（标准饮食、富含大米的饮食和富含大米和盐的饮食）的组合在5个实验组的小鼠尿液中，使所有雄激素、孕激素和皮质激素的排泄产生了多种变化。从5个实验组的尿液甾体数据中提取出一个共同的甾体特征——相对于四氢皮质醇，雄激素、孕激素和大多数皮质激素普遍降低。通过对照试验，发现psp-c和富含大米和盐的饮食预处理的组合是重现宫颈癌特异性尿液甾体变化的最佳选择。3）血浆甾体分析的结果虽然与尿液甾体数据不矛盾，但在本研究中是零碎的且信息量不大。根据包括维生素C的抗癌作用在内的相关信息，讨论了观察到的小鼠对致癌损伤的抗性以及蜕膜瘤在宫颈癌发生中的可能作用。
