Agarwal A, Ikemoto I, Loughlin K R
Division of Urology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Arch Androl. 1994 May-Jun;32(3):169-74. doi: 10.3109/01485019408987783.
In previous studies, high levels of reactive oxygen species (ROS) have been associated with an adverse impact on the fertilizing ability of sperm. The present study evaluated ROS levels in unprocessed (raw) semen specimens and in specimens processed by the traditional swim-up method and by L4 filtration, thereby assessing the potential of these techniques to damage sperm. Semen specimens from 26 men (10 patients in whom subfertility was suspected and 16 donors with normal fertility) were randomly collected, and ROS levels were measured. For all specimens combined (those from patients and those from donors), ROS levels were significantly lower after L4 filtration (6.07 +/- 1.97 mV/s 10(9) sperm-1, p < .01) and after swim-up (5.70 +/- 0.96 mV/s 10(9) sperm-1, p < .001) than in unprocessed ejaculate (12.88 +/- 2.32 mV/s 10(9) sperm-1). However, ROS levels in swim-up and L4 specimens were not statistically different from each other. It would appear that sperm processing by either swim-up or L4 filtration yields specimens with significantly lower levels of ROS than are found in unprocessed ejaculate.
在以往的研究中，高水平的活性氧（ROS）已被证实会对精子的受精能力产生不利影响。本研究评估了未处理（原始）精液标本、经传统上游法处理的标本以及经L4过滤处理的标本中的ROS水平，从而评估这些技术对精子造成损伤的可能性。随机收集了26名男性的精液标本（10名疑似亚生育力患者和16名生育能力正常的供者），并测量了ROS水平。对于所有合并的标本（来自患者和供者的标本），L4过滤后（6.07±1.97 mV/s 10⁹精子⁻¹，p<.01）和上游法处理后（5.70±0.96 mV/s 10⁹精子⁻¹，p<.001）的ROS水平均显著低于未处理的射精标本（12.88±2.32 mV/s 10⁹精子⁻¹）。然而，上游法处理标本和L4过滤标本中的ROS水平在统计学上并无差异。看来，无论是上游法还是L4过滤法处理精子，所得到的标本中ROS水平均显著低于未处理的射精标本。
