Henkel R, Schill W B
Center of Dermatology and Andrology, Justus Liebig University, Giessen, Germany.
Andrologia. 1998;30 Suppl 1:91-7. doi: 10.1111/j.1439-0272.1998.tb02832.x.
A total of 196 patients attending the Center of Dermatology and Andrology, Giessen, Germany, were examined for fertility problems. Polymorphonuclear elastase, reactive oxygen species (ROS) and the number of round and peroxidase-positive cells were investigated in addition to routine semen analysis. The ejaculates were also analysed before and after sperm separation by means of swim-up or glass wool filtration. In 20 cases of leukocytospermia, sperm concentration, motility, viability, production of reactive oxygen species, and the number of peroxidase-positive cells were evaluated before and after glass wool filtration. The results show that ROS production by viable spermatozoa is highly correlated with the concentration of PMN elastase and the number of both peroxidase-positive and round cells. Multiple regression analysis with motility as dependent parameter showed the number of round cells (n = 91; r = -0.332; P = 0.0030) to be the most important parameter affecting motility, while ROS mainly affects the viability of spermatozoa (n = 69; r = 0.250; P = 0.0107). In the case of leukocytospermia, glass wool filtration significantly reduced the number of peroxidase-positive cells and ROS production (P = 0.0098 and P = 0.0005, respectively). Receiver operating characteristic (ROC) curve analysis for ROS production in the ejaculate using a concentration of 1.000 ng ml-1 PMN elastase as decisive parameter resulted in a cut-off value of 49,489.9 counts 10(-7) viable spermatozoa. The statistical parameters were: Sensitivity: 63.2%, specificity: 100%, positive predictive value: 100%, negative predictive value: 36.1%.
共有196名前往德国吉森皮肤病与男科学中心就诊的患者接受了生育问题检查。除了进行常规精液分析外，还对多形核弹性蛋白酶、活性氧（ROS）以及圆形和过氧化物酶阳性细胞的数量进行了研究。此外，还对通过上浮法或玻璃棉过滤法进行精子分离前后的精液进行了分析。在20例白细胞精子症患者中，评估了玻璃棉过滤前后的精子浓度、活力、存活率、活性氧产生量以及过氧化物酶阳性细胞的数量。结果表明，活精子产生的ROS与PMN弹性蛋白酶的浓度以及过氧化物酶阳性细胞和圆形细胞的数量高度相关。以活力为因变量进行的多元回归分析表明，圆形细胞的数量（n = 91；r = -0.332；P = 0.0030）是影响活力的最重要参数，而ROS主要影响精子的存活率（n = 69；r = 0.250；P = 0.0107）。在白细胞精子症的情况下，玻璃棉过滤显著减少了过氧化物酶阳性细胞的数量和ROS的产生（分别为P = 0.0098和P = 0.0005）。以1.000 ng/ml PMN弹性蛋白酶的浓度作为决定性参数，对射精中ROS产生进行的受试者工作特征（ROC）曲线分析得出的临界值为49,489.9个计数/10^(-7)个活精子。统计参数为：敏感性：63.2%，特异性：100%，阳性预测值：100%，阴性预测值：36.1%。
