Agarwal A, Ikemoto I, Loughlin K R
Division of Urology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
J Urol. 1994 Jul;152(1):107-10. doi: 10.1016/s0022-5347(17)32829-x.
The levels of reactive oxygen species were compared in semen specimens from suspected subfertile men and from normal volunteers, and correlated with other semen parameters. Reactive oxygen species formation was measured in semen samples that had no white blood cells by a chemiluminescence assay with a Luminometer. The relationship of seminal reactive oxygen species to several sperm parameters was evaluated. A total of 84 specimens from 69 suspected subfertile men and 15 normal volunteers was tested for reactive oxygen species production. Comparison of reactive oxygen species levels in white blood cell-negative patient and donor specimens showed significantly higher values (p < 0.005) in the patient group. Similarly, levels in patient and donor specimens with normal sperm motility were significantly lower (p < 0.005) than those in specimens showing poor motility. The seminal reactive oxygen species levels of white blood cell-negative patients with abnormal morphology were significantly higher (p < 0.005) than those in white blood cell-negative patients with normal morphology. Our results show that seminal reactive oxygen species levels in suspected subfertile men are significantly higher than in normal men, and that the presence of excess reactive oxygen species in semen is positively correlated with low sperm concentration, poor motility and poor morphology. In conclusion, the evaluation of reactive oxygen species levels in cases of idiopathic male infertility could serve as an important marker of sperm dysfunction.
对疑似亚生育男性和正常志愿者的精液样本中的活性氧水平进行了比较，并与其他精液参数相关联。通过用光度计进行化学发光测定，在无白细胞的精液样本中测量活性氧的形成。评估了精液活性氧与几个精子参数的关系。对来自69名疑似亚生育男性和15名正常志愿者的总共84个样本进行了活性氧产生测试。白细胞阴性患者和供体样本中活性氧水平的比较显示，患者组的值明显更高（p < 0.005）。同样，精子活力正常的患者和供体样本中的水平明显低于活力差的样本（p < 0.005）。形态异常的白细胞阴性患者的精液活性氧水平明显高于形态正常的白细胞阴性患者（p < 0.005）。我们的结果表明，疑似亚生育男性的精液活性氧水平明显高于正常男性，并且精液中过量活性氧的存在与精子浓度低、活力差和形态差呈正相关。总之，对特发性男性不育病例中活性氧水平的评估可作为精子功能障碍的重要标志物。
