Han K, Hong J, Lim H C
Department of Chemical and Biochemical Engineering, University of California, Irvine 92717.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):640-5. doi: 10.1006/bbrc.1994.2230.
cAMP, ethanol, and CO2 production in starved yeast cells after the addition of D-glucose anomer was measured and compared over a wide range of anomer concentrations. At 1.0 g/l or higher concentrations, the addition of beta-D-glucose resulted in a higher cAMP peak. beta-D-glucose was more rapidly metabolized to ethanol and CO2 than alpha-D-glucose, although there was no notable difference in the uptake rates of the two anomers. At 0.4 g/l D-glucose anomer, the differences in cAMP and ethanol production rates for the two anomers were not significant. At 0.2 g/l D-glucose anomer or lower concentrations, ethanol production with alpha-D-glucose was higher than that with beta-D-glucose. The uptake rate of alpha-D-glucose was higher than that of beta-D-glucose at this low concentration.
在添加D-葡萄糖异头物后，对饥饿酵母细胞中的环磷酸腺苷（cAMP）、乙醇和二氧化碳产量进行了测量，并在广泛的异头物浓度范围内进行了比较。在1.0克/升或更高浓度下，添加β-D-葡萄糖会导致更高的cAMP峰值。β-D-葡萄糖比α-D-葡萄糖更快地代谢为乙醇和二氧化碳，尽管两种异头物的摄取速率没有显著差异。在0.4克/升D-葡萄糖异头物时，两种异头物的cAMP和乙醇产生速率差异不显著。在0.2克/升D-葡萄糖异头物或更低浓度下，α-D-葡萄糖的乙醇产量高于β-D-葡萄糖。在这种低浓度下，α-D-葡萄糖的摄取速率高于β-D-葡萄糖。
