Slater D, Berger L, Moore G, Bennett P
Action Research Laboratory for the Molecular Biology of Fetal Development, Royal Postgraduate Medical School, Queen Charlotte's Hospital, United Kingdom.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):67-71. doi: 10.1006/bbrc.1994.2149.
The onset of labour is associated with an increase in prostaglandin synthesis in amnion which appears to be mediated at least in part by an increase in cyclo-oxygenase (COX) expression. We have tested the hypothesis that COX expression is controlled in amnion by the binding of a protein to the COX mRNA which may inhibits its translation, as has been seen in vascular smooth muscle cells. Using differential RNA extraction protocols which extract either total mRNA or only mRNA which is not protein bound, we have found that, in amnion, the increase in COX-2 expression seen after the onset of labour is entirely in the protein bound fraction. Unlike in vascular smooth muscle, increased expression of COX-2 in amnion cells is therefore associated with increased rather than decreased protein binding to the mRNA. This protein may be involved in translation initiation or elongation or in mRNA stability. There does not appear to be a protein bound COX-1 mRNA fraction.
分娩的开始与羊膜中前列腺素合成的增加有关，这似乎至少部分是由环氧化酶（COX）表达的增加介导的。我们已经检验了这样一个假设，即COX的表达在羊膜中是通过一种蛋白质与COX mRNA的结合来控制的，这种结合可能会抑制其翻译，就像在血管平滑肌细胞中看到的那样。使用差异RNA提取方案，该方案可以提取总mRNA或仅提取未与蛋白质结合的mRNA，我们发现在羊膜中，分娩开始后COX-2表达的增加完全存在于与蛋白质结合的部分。与血管平滑肌不同，因此羊膜细胞中COX-2表达的增加与mRNA上蛋白质结合的增加而非减少有关。这种蛋白质可能参与翻译起始或延伸或mRNA稳定性。似乎不存在与蛋白质结合的COX-1 mRNA部分。
