Slater D, Berger L, Newton R, Moore G, Bennett P
Action Research Laboratory for the Molecular Biology of Fetal Development, Royal Postgraduate Medical School, London, UK.
Biochem Biophys Res Commun. 1994 Jan 14;198(1):304-8. doi: 10.1006/bbrc.1994.1043.
The onset of labor in humans is associated with an increase in prostaglandin production. One of the key steps is the conversion of arachidonic acid to prostaglandin E2 by cyclo-oxygenase (Cox). Cox has been found to exist as two distinct genes, Cox-1 and Cox-2. We have used RT-PCR to study the relative abundance of mRNA from each Cox gene in amnion at term. Quantitation of PCR efficiency indicated an approximate 100 fold excess of Cox-2 messenger RNA over that for Cox-1. These data point to the importance of Cox-2 in the increased prostaglandin synthesis associated with labor. Further studies should therefore focus on the control mechanisms for Cox-2.
人类分娩的开始与前列腺素生成增加有关。关键步骤之一是环氧化酶（Cox）将花生四烯酸转化为前列腺素E2。已发现Cox以两种不同的基因形式存在，即Cox-1和Cox-2。我们使用逆转录聚合酶链反应（RT-PCR）来研究足月羊膜中每个Cox基因的mRNA相对丰度。PCR效率的定量分析表明，Cox-2信使核糖核酸（mRNA）的量比Cox-1的量大约多出100倍。这些数据表明Cox-2在与分娩相关的前列腺素合成增加中具有重要作用。因此，进一步的研究应集中在Cox-2的调控机制上。
