Mitsui H, Nakatani K, Yamaguchi-Shinozaki K, Shinozaki K, Nishikawa K, Takahashi H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Plant Mol Biol. 1994 Aug;25(5):865-76. doi: 10.1007/BF00028881.
Complementary DNAs of two kinesin-related genes, katB and katC, were isolated from Arabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long alpha-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207-754)), which includes the carboxyl-terminal region of KatC, was expressed in Escherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.
从拟南芥中分离出两个与驱动蛋白相关的基因katB和katC的互补DNA并进行了测序。这些基因编码的多肽的羧基末端区域，特别是推测的ATP结合域和微管结合域，与驱动蛋白重链的机械化学马达域具有显著的序列同源性。KatB和KatC蛋白的预测二级结构包括羧基末端区域的一个大球状结构域和氨基末端区域的一个小球状结构域，它们被一个带有七肽重复序列的长α螺旋卷曲螺旋隔开。一种截短的KatC多肽（KatC(207-754)），其包含KatC的羧基末端区域，在大肠杆菌中表达，并显示具有微管刺激的ATP酶活性，并以ATP敏感的方式与微管结合，这两者都是驱动蛋白和驱动蛋白样蛋白的特征。
