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在同步化的烟草BY-2细胞中，驱动蛋白样蛋白KatB/C的细胞周期依赖性积累。

Cell cycle-dependent accumulation of a kinesin-like protein, KatB/C in synchronized tobacco BY-2 cells.

作者信息

Mitsui H, Hasezawa S, Nagata T, Takahashi H

机构信息

Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.

出版信息

Plant Mol Biol. 1996 Jan;30(1):177-81. doi: 10.1007/BF00017812.

DOI:10.1007/BF00017812
PMID:8616235
Abstract

Immunoblot analysis with antibodies prepared against highly purified recombinant truncated kinesin-like proteins, KatB(5-249) and KatC(207-754), encoded by the katB and katC genes of Arabidopsis thaliana revealed the presence of a kinesin-like polypeptide, termed KatB/C, in cultured tobacco BY-2 cells. The KatB/C polypeptide cosedimented with microtubules in the presence of a nonhydrolyzable ATP analogue and was released from microtubules in the presence of ATP, both of which are characteristics of kinesin proteins. The amount of KatB/C polypeptide in synchronous BY-2 cells increased during M phase of the cell cycle. Microtubule-based structures present in cells at M phase, such as the spindle and phragmoplast, may be the site of action of the KatB/C protein.

摘要

用针对由拟南芥katB和katC基因编码的高度纯化的重组截短驱动蛋白样蛋白KatB(5 - 249)和KatC(207 - 754)制备的抗体进行免疫印迹分析，结果显示在培养的烟草BY - 2细胞中存在一种驱动蛋白样多肽，称为KatB/C。在不可水解的ATP类似物存在下，KatB/C多肽与微管共沉降，并在ATP存在下从微管中释放，这两者都是驱动蛋白的特征。同步化的BY - 2细胞中KatB/C多肽的量在细胞周期的M期增加。M期细胞中存在的基于微管的结构，如纺锤体和成膜体，可能是KatB/C蛋白的作用位点。

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本文引用的文献

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Mitotic protoplasts and their infection with tobacco mosaic virus RNA encapsulated in liposomes.有丝分裂原生质体及其用脂质体包被的烟草花叶病毒 RNA 的感染。
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Identification and characterization of a novel microtubule-based motor associated with membranous organelles in tobacco pollen tubes.烟草花粉管中一种与膜性细胞器相关的新型微管马达的鉴定与特性分析
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The kinesin-like calmodulin binding protein is differentially involved in cell division.类驱动蛋白钙调蛋白结合蛋白在细胞分裂中发挥不同作用。
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向《一千零一夜》中的山鲁佐德致歉：关于1001个驱动蛋白分子的故事。
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