Hashimoto T, Frieben W R, Conti S F
J Bacteriol. 1969 Dec;100(3):1385-92. doi: 10.1128/jb.100.3.1385-1392.1969.
The biphasic nature of germination curves of individual Bacillus cereus T spores was further characterized by assessing the effects of temperature, concentration of germinants, and some inorganic cations on microgermination. Temperature was shown to affect both phases of microgermination as well as the microlag period, whereas the concentration of l-alanine and supplementation with adenosine exerted a significant effect only on the microlag period. The germination curves of individual spores induced by inosine were also biphasic and resembled those of spores induced by l-alanine. High concentrations (0.1 m or higher) of calcium and other inorganic cations prolonged both phases of microgermination, particularly the second phase, and had a less pronounced effect on the microlag period. The second phase of microgermination was completely inhibited when spores were germinated either in the presence of 0.3 m CaCl(2) or at a temperature of 43 C; this inhibition was reversible. Observations on the germination of spore suspensions (kinetics of the release of dipicolinic acid and mucopeptides, loss of heat resistance, increase in stainability, decrease in turbidity and refractility) were interpreted on the basis of the biphasic nature of microgermination. Dye uptake by individual spores during germination appeared also to be a biphasic process.
通过评估温度、萌发剂浓度和一些无机阳离子对微萌发的影响，进一步表征了单个蜡样芽孢杆菌T孢子萌发曲线的双相性质。结果表明，温度对微萌发的两个阶段以及微延迟期均有影响，而L-丙氨酸的浓度和腺苷的添加仅对微延迟期有显著影响。肌苷诱导的单个孢子的萌发曲线也是双相的，与L-丙氨酸诱导的孢子的萌发曲线相似。高浓度（0.1m或更高）的钙和其他无机阳离子延长了微萌发的两个阶段，特别是第二阶段，对微延迟期的影响较小。当孢子在0.3m CaCl₂存在下或在43℃温度下萌发时，微萌发的第二阶段被完全抑制；这种抑制是可逆的。基于微萌发的双相性质，对孢子悬浮液萌发的观察结果（吡啶二羧酸和粘肽释放的动力学、耐热性丧失、染色性增加、浊度和折光率降低）进行了解释。单个孢子在萌发过程中的染料摄取似乎也是一个双相过程。
