Schmitt K, Foster J W, Feakes R W, Knights C, Davis M E, Spillett D J, Goodfellow P N
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, United Kingdom.
Genomics. 1996 Jun 1;34(2):193-7. doi: 10.1006/geno.1996.0265.
Whole-genome radiation hybrids have been used to construct human genome maps that integrate different types of markers. To investigate this methodology in mammalian species other than humans, a panel of 164 mouse x hamster whole-genome radiation hybrids was constructed. This set of hybrids was used to produce a high-resolution map of a region on MMU11 that included microsatellite markers and cDNA sequences. The mouse homologue of the human SRY-related gene SOX9 was mapped to an interval of approximately 1.1 cM flanked by the microsatellite markers D11Mit11 and D11Mit291. This interval includes the region containing the mouse Tail-short mutation, a possible homologue of the human syndrome campomelic dysplasia, which is caused by mutations in SOX9. Our results suggest that whole-genome radiation hybrid technology will be a useful adjunct to mapping the genomes of nonhuman mammalian species.
全基因组辐射杂种细胞已被用于构建整合不同类型标记的人类基因组图谱。为了在人类以外的哺乳动物物种中研究这种方法，构建了一组由164个小鼠×仓鼠全基因组辐射杂种细胞组成的细胞系。这组杂种细胞被用于绘制MMU11上一个区域的高分辨率图谱，该区域包括微卫星标记和cDNA序列。人类SRY相关基因SOX9的小鼠同源基因被定位到一个约1.1 cM的区间，两侧是微卫星标记D11Mit11和D11Mit291。这个区间包括含有小鼠短尾突变的区域，它可能是人类综合征性脊柱发育不良的同源物，该综合征由SOX9突变引起。我们的结果表明，全基因组辐射杂种细胞技术将是绘制非人类哺乳动物物种基因组图谱的有用辅助手段。
