Langston A A, Mellersh C S, Neal C L, Ray K, Acland G M, Gibbs M, Aguirre G D, Fournier R E, Ostrander E A
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Genomics. 1997 Dec 15;46(3):317-25. doi: 10.1006/geno.1997.5009.
We have constructed a collection of canine-rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescence in situ hybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.
我们通过将犬成纤维细胞微细胞供体与永生化啮齿动物受体细胞融合，构建了一组犬-啮齿动物微细胞杂交细胞系。使用荧光原位杂交和犬微卫星重复序列的PCR分析相结合的方法对杂交细胞系进行表征，从而筛选出一组杂种，其中大多数犬染色体都有代表。基于杂种集中常见的保留模式，大约90%经过测试的遗传标记和基因可以被分配到31个匿名犬染色体组中的一个。现在，许多这些假定的染色体组已通过连锁分析得到验证。这组细胞系为犬基因组的遗传、物理和比较图谱的开发提供了一个工具。
