Talaska G, Schamer M, Casetta G, Tizzani A, Vineis P
Department of Environmental Health, University of Cincinnati, OH.
Cancer Lett. 1994 Aug 29;84(1):93-7. doi: 10.1016/0304-3835(94)90362-x.
We have determined the levels of DNA adducts (using 32P-postlabelling) in the biopsies of 20 bladder cancer patients and in the exfoliated bladder cells of 36 healthy volunteers. The aims of the study were (a) to estimate the concentration of DNA adducts in cancer cases and controls according to the level of smoking; and (b) to investigate whether bladder cancer cases had higher levels of adducts in bladder cells than healthy controls had. A dose-response relationship between smoking levels and adduct levels was present among both cancer cases and controls. Cancer cases and the controls had similar adduct levels for the same level of smoking. According to a risk assessment exercise, adduct levels among heavy smokers were roughly comparable with those found in mice and dogs treated with bladder carcinogens, at doses which induce a 50% lifetime risk of bladder cancer.
我们已经测定了20名膀胱癌患者活检组织以及36名健康志愿者膀胱脱落细胞中的DNA加合物水平（采用32P后标记法）。本研究的目的是：（a）根据吸烟水平估计癌症患者和对照者中DNA加合物的浓度；（b）调查膀胱癌患者膀胱细胞中的加合物水平是否高于健康对照者。在癌症患者和对照者中，吸烟水平与加合物水平之间均存在剂量反应关系。对于相同的吸烟水平，癌症患者和对照者的加合物水平相似。根据一项风险评估活动，重度吸烟者的加合物水平与用膀胱致癌物处理的小鼠和狗在诱导膀胱癌终生风险为50%的剂量下所发现的加合物水平大致相当。
