Evans J, Smith M A, Steel C M
J Immunol Methods. 1975 Jul;7(4):371-8. doi: 10.1016/0022-1759(75)90045-9.
Blood mononuclear cells from ten healthy donors and cells from fifty cultured human lymphoblastoid lines were tested for the capacity to form rosettes with sheep erythrocytes. The effect of adding glutaralhehyde before scoring rosettes and the effect of substituting glutaraldehyde-fixed for fresh sheep cells were both examined. The results suggest that glutaraldehyde increases the apparent proportion of rosette-forming cells by altering the sheep erythrocytes in such a way that they will adhere to some B lymphocytes. Although a small number of cells carry both surface immunoglobulins and receptors for fresh sheep erythrocytes, the rosette test with fresh sheep cells identifies T lymphocytes more accurately in the absence of glutaraldehyde than in its presence.
对来自10名健康供体的血液单核细胞以及50株培养的人淋巴母细胞系的细胞进行了检测，以评估其与绵羊红细胞形成玫瑰花结的能力。研究了在计数玫瑰花结之前添加戊二醛的效果以及用戊二醛固定的绵羊细胞替代新鲜绵羊细胞的效果。结果表明，戊二醛通过改变绵羊红细胞，使其能够黏附于一些B淋巴细胞，从而增加了形成玫瑰花结细胞的表观比例。尽管少数细胞同时携带表面免疫球蛋白和新鲜绵羊红细胞受体，但在没有戊二醛的情况下，用新鲜绵羊细胞进行的玫瑰花结试验比有戊二醛时更能准确地识别T淋巴细胞。
