Dolgushin I I, Portnaia L V
Zh Mikrobiol Epidemiol Immunobiol. 1977 Oct(10):87-91.
The authors analysed the influence of some conditions of studying the rosette-formation between the human peripheral blood lymphocytes and sheep red blood cells, not loaded (a) and loaded with antibodies and a complement (b). It was shown that rosette formation depended on the temperature at which the experiment was conducted. Thus, after the incubation of a mixture of cells at 37 degrees C, and then at 4 degrees C the number of rosette-forming cells was greater than after the incubation at 4 degrees C alone. Substances used for their fixation also influenced the formation of rosettes: in the treatment of erythrocytes with formalin, tannin and glutaraldehyde, as well as introduction of the latter into the mixture of erythrocytes and leukocytes considerably increased the number of a and b (the sum of T- and B-lymphocytes exceeded 100%). Among a and b in such experimental conditions there was seen a great number of monocytes and of segmented leukocytes. Fixation of smears prepared from a mixture of cells after the incubation in formalin vapour did not lead to such increase in the number of rosette-forming cells. Apparently in case of using glutardehyde, formalin and tannin cells for the treatment of cells additional investigations are necessary with the purpose of identification of a and b as T- and B-lymphocytes.
作者分析了某些研究人外周血淋巴细胞与未负载（a）以及负载抗体和补体（b）的绵羊红细胞之间花环形成的条件的影响。结果表明，花环形成取决于进行实验的温度。因此，细胞混合物在37℃孵育后再在4℃孵育，形成花环的细胞数量比仅在4℃孵育后的要多。用于固定细胞的物质也影响花环的形成：用福尔马林、单宁和戊二醛处理红细胞，以及将戊二醛引入红细胞和白细胞的混合物中，显著增加了a和b的数量（T淋巴细胞和B淋巴细胞的总和超过了100%）。在这种实验条件下的a和b中，可见大量单核细胞和分叶白细胞。用福尔马林蒸汽孵育后由细胞混合物制备的涂片固定后，形成花环的细胞数量并未增加。显然，在用戊二醛、福尔马林和单宁处理细胞的情况下，有必要进行额外的研究，以将a和b鉴定为T淋巴细胞和B淋巴细胞。
