乙二胺四乙酸和乙二醇双（β-氨基乙醚）-N，N-四乙酸对法德巴克淋球菌试验凝集反应的增强作用
Enhancement of coagglutination reactions of the Phadebact gonococcus test by ethylenediaminetetraacetate and ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetate.
作者信息
Izakson I, Morse S A
出版信息
J Clin Microbiol. 1981 Sep;14(3):261-5. doi: 10.1128/jcm.14.3.261-265.1981.
Abstract
Incubation of gonococci under conditions optimal for autolysis resulted in increased sensitivity and enhancement of the coagglutination reaction of the Phadebact gonococcus test. These conditions included an alkaline pH (pH 8.3) and the presence of divalent cation chelators such as ethylenediaminetetraacetic acid or ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid. Heating cell suspensions at 90 degrees C for 15 min before assay by coagglutination produced a further increase in sensitivity and enhancement of the reaction. Gonococcal lipopolysaccharide was found to be an important antigen in these coagglutination reactions. The detection of lipopolysaccharide was markedly enhanced by the addition of chelating agents.
摘要
在最适合自溶的条件下培养淋球菌，会提高其敏感性，并增强法代巴克特淋球菌试验的协同凝集反应。这些条件包括碱性pH值（pH 8.3）以及存在二价阳离子螯合剂，如乙二胺四乙酸或乙二醇双（β-氨基乙醚）-N,N-四乙酸。在通过协同凝集法检测前，将细胞悬液在90℃加热15分钟，会进一步提高敏感性并增强反应。发现淋球菌脂多糖是这些协同凝集反应中的重要抗原。添加螯合剂可显著增强脂多糖的检测。
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