Treichel U, McFarlane B M, Seki T, Krawitt E L, Alessi N, Stickel F, McFarlane I G, Kiyosawa K, Furuta S, Freni M A
Department of Medicine, Johannes Gutenberg University, Mainz, Germany.
Gastroenterology. 1994 Sep;107(3):799-804. doi: 10.1016/0016-5085(94)90129-5.
BACKGROUND/AIMS: The asialoglycoprotein receptor (ASGPR) is an established, liver-specific autoantigen. This multicenter study investigated the specificity of anti-ASGPR autoantibodies for autoimmune hepatitis (AIH) in different ethnic groups.
Nine hundred fourteen sera from European, Japanese, and North American (U.S.) patients with chronic inflammatory liver disorders were tested. An enzyme-immunoassay using human ASGPR and a radioimmunoassay against rabbit ASGPR, performed independently on coded sera, were compared.
The highest frequency (76%) of anti-human ASGPR was found in AIH patients (11/24 U.S.; 21/25 European; 28/30 Japanese), particularly in those with active disease before treatment (53/62, 85%), and decreased in titer with response to immunosuppressive therapy. These antibodies were found at low titers in 43 (11%) of 385 patients with viral hepatitis and in 25 (7.6%) of 328 patients with other chronic inflammatory liver disorders (P < 0.0005 compared with all AIH patients). Twenty of 37 sera tested by enzyme-immunoassay and radioimmunoassay were positive, and nine were negative for anti-ASGPR by both assays (78% concordance); six sera were exclusively positive on human substrate.
Circulating anti-ASGPR autoantibodies are closely associated with autoimmune hepatitis independent of geographic or ethnic criteria. Two anti-ASGPR assays currently in use show high reliability.
背景/目的：去唾液酸糖蛋白受体（ASGPR）是一种公认的肝脏特异性自身抗原。这项多中心研究调查了不同种族中抗ASGPR自身抗体对自身免疫性肝炎（AIH）的特异性。
检测了来自欧洲、日本和北美（美国）慢性炎症性肝病患者的914份血清。对使用人ASGPR的酶免疫测定和针对兔ASGPR的放射免疫测定进行了比较，这两种测定均对编码血清独立进行。
抗人ASGPR的最高频率（76%）见于AIH患者（美国患者中24例有11例；欧洲患者中25例有21例；日本患者中30例有28例），尤其见于治疗前疾病活动的患者（62例中有53例，85%），且随着免疫抑制治疗的反应其滴度下降。在385例病毒性肝炎患者中有43例（11%）以及328例其他慢性炎症性肝病患者中有25例（7.6%）发现这些抗体滴度较低（与所有AIH患者相比，P<0.0005）。通过酶免疫测定和放射免疫测定检测的37份血清中有20份呈阳性，两种测定均显示9份抗ASGPR阴性（一致性为78%）；6份血清仅在人底物上呈阳性。
循环抗ASGPR自身抗体与自身免疫性肝炎密切相关，与地理或种族标准无关。目前使用的两种抗ASGPR测定显示出高可靠性。
