Sutrave P, Shafer B K, Strathern J N, Hughes S H
ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
Gene. 1994 Sep 2;146(2):209-13. doi: 10.1016/0378-1119(94)90294-1.
We have cloned and characterized the FUN12 gene which is found on chromosome 1 of Saccharomyces cerevisiae. The complete nucleotide (nt) sequence of the cDNA and the genomic clones shows that FUN12 is expressed as a 3.7-kb message and should encode a 97 kDa-protein. Immunoprecipitations using antipeptide antibodies showed that the cells contain a Fun12p of this size. The databases contain no nt sequences that are homologous to FUN12 and no protein homologous to Fun12p. Gene disruption experiments showed that FUN12 is an essential gene.
我们已经克隆并鉴定了在酿酒酵母1号染色体上发现的FUN12基因。cDNA和基因组克隆的完整核苷酸（nt）序列表明，FUN12以3.7 kb的信使RNA形式表达，应该编码一种97 kDa的蛋白质。使用抗肽抗体进行的免疫沉淀表明，细胞中含有这种大小的Fun12p。数据库中没有与FUN12同源的核苷酸序列，也没有与Fun12p同源的蛋白质。基因破坏实验表明，FUN12是一个必需基因。
