Li G Y, Tian G L, Slonimski P P, Herbert C J
Centre de Génétique Moléculaire, Laboratoire propre du CNRS, Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Mol Gen Genet. 1996 Feb 25;250(3):316-22. doi: 10.1007/BF02174389.
In Saccharomyces cerevisiae the only known role of the CBP2 gene is the excision of the fifth intron of the mitochondrial cyt b gene (bI5). We have cloned the CBP2 gene from Saccharomyces douglasii (a close relative of S. cerevisiae). A comparison of the S. douglasii and S. cerevisiae sequences shows that there are 14% nucleotide substitutions in the coding region, with transitions being three times more frequent than transversions. At the protein level sequence identity is 87%. We have demonstrated that the S. douglasii CBP2 gene is essential for respiratory growth in the presence of a wild-type S. douglasii mitochondrial genome, but not in the presence of an intronless S. cerevisiae mitochondrial genome. Also the S. douglasii and S. cerevisiae CBP2 genes are completely interchangeable, even though the intron bI5 is absent from the S. douglasii mitochondrial genome.
在酿酒酵母中，CBP2基因唯一已知的作用是切除线粒体细胞色素b基因（bI5）的第五个内含子。我们从道格拉斯酿酒酵母（酿酒酵母的近亲）中克隆了CBP2基因。对道格拉斯酿酒酵母和酿酒酵母序列的比较表明，编码区有14%的核苷酸替换，转换的频率是颠换的三倍。在蛋白质水平上，序列同一性为87%。我们已经证明，在存在野生型道格拉斯酿酒酵母线粒体基因组的情况下，道格拉斯酿酒酵母CBP2基因对于呼吸生长是必需的，但在无内含子的酿酒酵母线粒体基因组存在的情况下则不是。此外，道格拉斯酿酒酵母和酿酒酵母的CBP2基因是完全可互换的，尽管道格拉斯酿酒酵母线粒体基因组中不存在内含子bI5。
