百日咳博德特氏菌在三种温度下于四种悬浮液中的生存能力。
Viability of Bordetella pertussis in four suspending solutions at three temperatures.
作者信息
Cassiday P K, Sanden G N, Kane C T, M'Boup S, Barbaree J M
机构信息
Childhood and Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1994 Jun;32(6):1550-3. doi: 10.1128/jcm.32.6.1550-1553.1994.
We studied the survival of Bordetella pertussis in four suspending solutions (Casamino Acids broth, deionized water, phosphate-buffered saline, and serum inositol), subjected to three storage temperatures (4, -20, and -70 degrees C) and two freezing methods (direct freezing and fast-freezing in an ethanol-dry-ice bath). Recovery rates were higher for longer periods for suspensions stored at -70 degrees C than those stored at -20 or 4 degrees C. Serum inositol showed the highest recovery rates for all experimental conditions, followed by Casamino Acids, deionized water, and phosphate-buffered saline. Cell viability was significantly reduced in phosphate-buffered saline suspensions fast-frozen before storage. These results identify optimal conditions for storing B. pertussis cells and are applicable to the collection, transport, and storage of aspirated nasopharyngeal samples for use in the laboratory diagnosis of pertussis.
我们研究了百日咳博德特氏菌在四种悬浮液（酪蛋白氨基酸肉汤、去离子水、磷酸盐缓冲盐水和血清肌醇）中的存活情况，这些悬浮液分别经受三种储存温度（4℃、-20℃和-70℃）以及两种冷冻方法（直接冷冻和在乙醇-干冰浴中快速冷冻）。在-70℃储存的悬浮液在较长时间内的回收率高于在-20℃或4℃储存的悬浮液。在所有实验条件下，血清肌醇的回收率最高，其次是酪蛋白氨基酸、去离子水和磷酸盐缓冲盐水。储存前快速冷冻的磷酸盐缓冲盐水悬浮液中的细胞活力显著降低。这些结果确定了储存百日咳博德特氏菌细胞的最佳条件，适用于采集、运输和储存用于百日咳实验室诊断的吸出鼻咽样本。
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