Komuro T, Shimizu D, Hashimoto Y, Takeuchi K, Nango N
Department of Basic Human Sciences, School of Human Sciences, Waseda University, Saitama, Japan.
J Electron Microsc (Tokyo). 1994 Apr;43(2):72-6.
A new morphometry system for submicroscopic three-dimensional structures was developed on the same principles as for the aerial survey using SEM stereo-pairs. This computer graphic system was applied to the analysis of the surface features of the intestinal mucosa. After removing the epithelial layer by prolonged osmication, core structures of the intestinal villi were exposed and 100 villi each from rat and mouse were measured. Average height, basal area and surface area of a single core of the rat were 188 microns, 20,200 microns2 and 93,500 microns2, respectively. Those of the mouse were 148 microns, 7,530 microns2 and 37,100 microns2, respectively. Surface amplifications by the rat and the mouse villi, after compensating for epithelial thickness, were calculated as 3.5 and 4.3, respectively; though amplifications based on the villous core were 4.6 and 4.9, respectively. These three-dimensional features of the intestinal mucosa were illustrated as submicroscopic maps by drawing their contour lines. It was demonstrated that the present morphometry system makes it possible to know not only the average features of a large sample, but also a variety of features of individual structures which were directly observed under SEM.
一种用于亚微观三维结构的新形态测量系统，是基于与使用扫描电子显微镜（SEM）立体对进行航空测量相同的原理开发的。该计算机图形系统应用于肠道黏膜表面特征的分析。通过长时间的锇酸固定去除上皮层后，暴露了肠绒毛的核心结构，并对大鼠和小鼠各100个绒毛进行了测量。大鼠单个核心的平均高度、基部面积和表面积分别为188微米、20200平方微米和93500平方微米。小鼠的分别为148微米、7530平方微米和37100平方微米。在补偿上皮厚度后，大鼠和小鼠绒毛的表面放大倍数分别计算为3.5和4.3；基于绒毛核心的放大倍数分别为4.6和4.9。通过绘制轮廓线，将肠道黏膜的这些三维特征绘制成亚微观图谱。结果表明，目前的形态测量系统不仅能够了解大样本的平均特征，还能够了解在扫描电子显微镜下直接观察到的单个结构的各种特征。
