通过改良的非洲爪蟾胚胎致畸试验（FETAX）程序测定庚醇对非洲爪蟾的致死性、致畸性和生长抑制作用。

Lethality, teratogenicity and growth inhibition of heptanol in Xenopus assayed by a modified frog embryo teratogenesis assay-Xenopus (FETAX) procedure.

作者信息

Bernardini G, Vismara C, Boracchi P, Camatini M

机构信息

Dipartimento di Biologia, Università di Milano, Italy.

出版信息

Sci Total Environ. 1994 Jul 4;151(1):1-8. doi: 10.1016/0048-9697(94)90480-4.

DOI:10.1016/0048-9697(94)90480-4

PMID:8079149

Abstract

The frog embryo teratogenesis assay-Xenopus (FETAX), a powerful test for the presence of developmental toxicants, has been modified mainly by performing an in vitro fertilization and increasing the exposure time to 112 h. The modified assay (modFETAX) that presents several advantages over the original FETAX methodology has been validated by the use of ZnSO4, a standard teratogen for FETAX. The modFETAX has been applied to evaluate the 1-heptanol effects on mortality, malformation and growth inhibition. The results indicate that heptanol causes a significant growth inhibition of Xenopus tadpoles and that LC50 and TC50 at 120 h are, respectively, 1.49 and 0.37 mM; the resulting teratogenic index (TI50) of 4.03 suggests that heptanol is a strong teratogen.

摘要

非洲爪蟾胚胎致畸试验（FETAX）是一种检测发育毒性物质的有效方法，主要通过进行体外受精并将暴露时间延长至112小时进行了改进。改进后的试验（modFETAX）相对于原始FETAX方法具有多个优点，已通过使用硫酸锌（FETAX的标准致畸剂）进行了验证。modFETAX已用于评估正庚醇对死亡率、畸形和生长抑制的影响。结果表明，正庚醇对非洲爪蟾蝌蚪有显著的生长抑制作用，120小时时的半数致死浓度（LC50）和半数致畸浓度（TC50）分别为1.49 mM和0.37 mM；由此产生的致畸指数（TI50）为4.03，表明正庚醇是一种强致畸剂。