Král J, Bastár M, Horyna B
Vet Med (Praha). 1975 Feb;20(02):83-9.
For precision of bacteriological diagnosis of Listeria monocytogenes (L. m.) the immune fluorescent technique (IFT) was assessed. On using the described immunization pattern high titres of specific antibodies were obtained from the immunized rabbits. The obtained hyperimmune sera yielded very good results when used in IFT, and they were suitable for conjugate production. By means of IFT L.M. was demonstrated from cultures, organ replicas and tissue sections of experimentally infected rabbits. The most brilliant fluorescence was achieved with conjugate of the corresponding serotype, the polytype conjugate yielded demonstrable fluorescence with all serotypes. Listerias showed intensive fluorescence, and in contrast staining of replicas and tissue sections with Evans blue they could be well differentiated from the background.
为了精确诊断单核细胞增生李斯特菌（L. m.），对免疫荧光技术（IFT）进行了评估。采用所述免疫模式，从免疫兔中获得了高滴度的特异性抗体。所获得的超免疫血清在免疫荧光技术中使用时产生了非常好的结果，并且适用于制备共轭物。通过免疫荧光技术，在实验感染兔的培养物、器官印模和组织切片中证实了单核细胞增生李斯特菌的存在。使用相应血清型的共轭物可获得最明亮的荧光，多型共轭物对所有血清型均产生可证实的荧光。李斯特菌呈现强烈荧光，与伊文思蓝对印模和组织切片的染色形成对比，它们能与背景很好地区分。
