Kotitschke K, Jung H, Nekolla S, Haase A, Bauer A, Bogdahn U
Institute of Physics, University of Wuerzburg, Germany.
NMR Biomed. 1994 May;7(3):111-20. doi: 10.1002/nbm.1940070303.
Astrocytoma (WHO grade II, III), glioblastoma, malignant melanoma, and normal glial cell cultures, established from biopsies, were investigated by 1H MRS. At a 1H resonance frequency of 500 MHz (11.75 T) a high spectral resolution was achieved in 1D 1H spectra; in conjunction with 2D shift-correlated (COSY) MRS, resonances of alanine, aspartate, choline, creatine, glutamate, glutamine, hypotaurine, myo-inositol, phosphocreatine, phosphoryl-ethanolamine, phosphoryl-choline, lactate, lysine, N-acetylaspartate, taurine, threonine and valine could be identified. T1 relaxation times for the most prominent compounds are presented. T1 values of lactate ranged between 450 ms and 850 ms. The intensity of the lactate signal revealed differences between individual spectra, but exhibited no correlation between different tumor specimens or degree of malignancy. It was shown that the lactate signal at 1.3 ppm is covered by peaks arising from threonine and fatty acids. The choline signal level varied among spectra of different tumors, among tumors with similar degree of malignancy, and within the same tumor. Further preliminary differences due to aspartate, inositol and glutamine/glutamate were found in 1D and 2D COSY spectra between normal glial cells as well as different tumors. These results indicate that some differences observed in in vivo spectra may be attributable to secondary macroscopic structural changes (hypoxia, necrosis) and not to tumor inherent characteristics. Further correlation between in vivo and in vitro spectroscopy is therefore required.
通过1H磁共振波谱（1H MRS）对星形细胞瘤（世界卫生组织二级、三级）、胶质母细胞瘤、恶性黑色素瘤以及取自活检样本建立的正常神经胶质细胞培养物进行了研究。在500 MHz（11.75 T）的1H共振频率下，一维1H谱实现了高光谱分辨率；结合二维位移相关（COSY）磁共振波谱，可识别出丙氨酸、天冬氨酸、胆碱、肌酸、谷氨酸、谷氨酰胺、亚牛磺酸、肌醇、磷酸肌酸、磷酸乙醇胺、磷酸胆碱、乳酸、赖氨酸、N - 乙酰天门冬氨酸、牛磺酸、苏氨酸和缬氨酸的共振峰。给出了最主要化合物的T1弛豫时间。乳酸的T1值在450毫秒至850毫秒之间。乳酸信号强度在各个谱图之间存在差异，但在不同肿瘤样本或恶性程度之间未显示出相关性。结果表明，1.3 ppm处的乳酸信号被苏氨酸和脂肪酸产生的峰所覆盖。胆碱信号水平在不同肿瘤的谱图之间、恶性程度相似的肿瘤之间以及同一肿瘤内部均有所不同。在正常神经胶质细胞以及不同肿瘤的一维和二维COSY谱中，还发现了天冬氨酸、肌醇和谷氨酰胺/谷氨酸的进一步初步差异。这些结果表明，体内谱图中观察到的一些差异可能归因于继发性宏观结构变化（缺氧、坏死），而非肿瘤固有的特征。因此，需要进一步研究体内和体外光谱学之间的相关性。
